LLPS-Hos-2406
RC3H1

▼ OVERVIEW

Status: Reviewed
Protein Name: Roquin-1
Gene Name: RC3H1, KIAA2025, RNF198
Ensembl Gene: ENSG00000135870.11
Ensembl Protein: ENSP00000356667.2
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: Client
PDB: 4QIK (A, C) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
P-body
"...Analysis of correlated patterns between endogenous preys uncovers the spatial organization of RNA regulatory structures and enables the definition of 144 core components of SGs and PBs."
N/A29395067
Stress granule
"...By adding known stress granule components not identified by mass spectrometry to this list, we created the first stress granule proteome for a mammalian cell."
U2OS cells26777405
Stress granule
"...Together, our APEX and IF screening approaches identified 260 SG proteins, including ~150 candidates that had not previously been associated with SGs."
Neural progenitor cells29373831
Stress granule
"...283 proteins identified as APEX hits in HEK293T cells or previously shown to associate with SGs."
N/A29373831
Stress granule
"...Analysis of correlated patterns between endogenous preys uncovers the spatial organization of RNA regulatory structures and enables the definition of 144 core components of SGs and PBs."
N/A29395067
Stress granule
"...A list of candidate SG proteins identified by mass spectrometry in NPCs, including statistical analysis."
Neural progenitor cells29373831

▼ FUNCTION

Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF, TNFRSF4 and in many more mRNAs (PubMed:25026078). Cleaves translationally inactive mRNAs harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-independent manner (By similarity). Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs (By similarity). In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity (By similarity). In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression (By similarity). Also recognizes CDE in its own mRNA and in that of paralogous RC3H2, possibly leading to feedback loop regulation (By similarity). Recognizes and binds mRNAs containing an hexaloop stem-loop motif, called alternative decay element (ADE) (By similarity). Able to interact with double-stranded RNA (dsRNA) (PubMed:25504471, PubMed:25026078). miRNA-binding protein that regulates microRNA homeostasis. Enhances DICER-mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression (PubMed:25697406). Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2A, UBE2B, UBE2D2, UBE2F, UBE2G1, UBE2G2 and UBE2L3 and produces polyubiquitin chains (PubMed:26489670). Show the strongest activity when paired with UBE2N:UBE2V1 or UBE2N:UBE2V2 E2 complexes and generate both short and long polyubiquitin chains (PubMed:26489670).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MPVQAPQWTD  FLSCPICTQT  FDETIRKPIS  LGCGHTVCKM  CLNKLHRKAC  PFDQTTINTD  60
61    IELLPVNSAL  LQLVGAQVPE  QQPITLCSGV  EDTKHYEEAK  KCVEELALYL  KPLSSARGVG  120
121   LNSTTQSVLS  RPMQRKLVTL  VHCQLVEEEG  RIRAMRAARS  LGERTVTELI  LQHQNPQQLS  180
181   SNLWAAVRAR  GCQFLGPAMQ  EEALKLVLLA  LEDGSALSRK  VLVLFVVQRL  EPRFPQASKT  240
241   SIGHVVQLLY  RASCFKVTKR  DEDSSLMQLK  EEFRTYEALR  REHDSQIVQI  AMEAGLRIAP  300
301   DQWSSLLYGD  QSHKSHMQSI  IDKLQTPASF  AQSVQELTIA  LQRTGDPANL  NRLRPHLELL  360
361   ANIDPSPDAP  PPTWEQLENG  LVAVRTVVHG  LVDYIQNHSK  KGADQQQPPQ  HSKYKTYMCR  420
421   DMKQRGGCPR  GASCTFAHSQ  EELEKFRKMN  KRLVPRRPLS  ASLGQLNEVG  LPSAAILPDE  480
481   GAVDLPSRKP  PALPNGIVST  GNTVTQLIPR  GTDPSYDSSL  KPGKIDHLSS  SAPGSPPDLL  540
541   ESVPKSISAL  PVNPHSIPPR  GPADLPPMPV  TKPLQMVPRG  SQLYPAQQTD  VYYQDPRGAA  600
601   PPFEPAPYQQ  GMYYTPPPQC  VSRFVRPPPS  APEPAPPYLD  HYPPYLQERV  VNSQYGTQPQ  660
661   QYPPIYPSHY  DGRRVYPAPS  YTREEIFRES  PIPIEIPPAA  VPSYVPESRE  RYQQIESYYP  720
721   VAPHPTQIRP  SYLREPPYSR  LPPPPQPHPS  LDELHRRRKE  IMAQLEERKV  ISPPPFAPSP  780
781   TLPPTFHPEE  FLDEDLKVAG  KYKGNDYSQY  SPWSCDTIGS  YIGTKDAKPK  DVVAAGSVEM  840
841   MNVESKGMRD  QRLDLQRRAA  ETSDDDLIPF  GDRPTVSRFG  AISRTSKTIY  QGAGPMQAMA  900
901   PQGAPTKSIN  ISDYSPYGTH  GGWGASPYSP  HQNIPSQGHF  SERERISMSE  VASHGKPLPS  960
961   AEREQLRLEL  QQLNHQISQQ  TQLRGLEAVS  NRLVLQREAN  TLAGQSQPPP  PPPPKWPGMI  1020
1021  SSEQLSLELH  QVEREIGKRT  RELSMENQCS  LDMKSKLNTS  KQAENGQPEP  QNKVPAEDLT  1080
1081  LTFSDVPNGS  ALTQENISLL  SNKTSSLNLS  EDPEGGGDNN  DSQRSGVTPS  SAP  1133
Nucleotide CDS Sequence (FASTA)
1     ATGCCTGTAC  AAGCTCCACA  ATGGACGGAT  TTCCTCTCCT  GCCCAATTTG  CACTCAGACT  60
61    TTCGACGAAA  CAATTCGAAA  GCCCATCAGT  TTGGGTTGTG  GCCATACTGT  CTGCAAGATG  120
121   TGCCTGAATA  AACTCCACCG  CAAGGCTTGC  CCATTTGACC  AGACCACTAT  CAATACAGAC  180
181   ATTGAGCTCC  TCCCTGTGAA  CTCAGCATTG  CTGCAGCTCG  TGGGTGCTCA  GGTCCCTGAG  240
241   CAGCAGCCTA  TTACTTTGTG  TAGTGGGGTT  GAAGACACAA  AGCATTATGA  GGAAGCCAAG  300
301   AAATGTGTAG  AAGAATTAGC  ATTGTACTTA  AAACCGCTCA  GCAGTGCTAG  AGGAGTGGGT  360
361   CTGAACAGCA  CTACTCAGAG  TGTTCTGAGT  CGCCCAATGC  AGAGGAAGCT  TGTGACTCTA  420
421   GTCCACTGTC  AACTAGTAGA  AGAAGAAGGC  AGGATTCGTG  CCATGAGGGC  AGCTCGATCT  480
481   TTAGGTGAAC  GAACAGTTAC  AGAGCTCATT  CTCCAGCACC  AGAATCCTCA  GCAACTCTCT  540
541   TCCAATCTTT  GGGCAGCAGT  AAGGGCTAGG  GGATGCCAGT  TCCTTGGACC  AGCAATGCAG  600
601   GAGGAAGCTT  TAAAGTTGGT  TCTGCTGGCC  TTAGAAGATG  GTTCTGCTTT  GTCAAGAAAA  660
661   GTATTGGTTC  TGTTTGTGGT  GCAAAGATTG  GAGCCACGGT  TTCCTCAAGC  CTCTAAAACT  720
721   AGCATTGGGC  ATGTTGTTCA  GCTCCTTTAT  AGAGCCTCCT  GTTTCAAGGT  CACCAAACGT  780
781   GATGAAGACT  CTTCTTTGAT  GCAGCTGAAA  GAAGAATTTA  GAACCTATGA  AGCTCTGCGG  840
841   CGAGAACATG  ACTCCCAGAT  AGTGCAGATT  GCTATGGAAG  CAGGCTTACG  AATTGCACCG  900
901   GACCAGTGGT  CCTCTTTGCT  TTATGGAGAC  CAGTCTCACA  AATCTCATAT  GCAGTCCATT  960
961   ATTGACAAGT  TGCAGACTCC  AGCCTCTTTT  GCACAGAGTG  TTCAGGAACT  AACAATTGCT  1020
1021  CTCCAGCGAA  CTGGAGACCC  AGCAAACTTG  AACCGACTAA  GACCCCATTT  GGAGCTGTTA  1080
1081  GCAAACATTG  ACCCTAGTCC  AGATGCTCCT  CCTCCTACTT  GGGAACAGCT  GGAAAATGGG  1140
1141  CTGGTTGCTG  TGCGTACAGT  GGTACATGGG  CTGGTTGATT  ATATCCAGAA  CCACAGCAAA  1200
1201  AAAGGAGCAG  ATCAGCAGCA  GCCTCCACAG  CATAGCAAAT  ACAAAACATA  CATGTGTCGA  1260
1261  GATATGAAGC  AGAGAGGAGG  ATGCCCTCGT  GGGGCCAGCT  GTACATTTGC  ACACTCACAG  1320
1321  GAGGAACTGG  AAAAATTTCG  TAAAATGAAT  AAGCGCCTGG  TTCCGAGAAG  ACCCTTGAGT  1380
1381  GCCTCTTTGG  GTCAACTTAA  TGAGGTGGGC  CTGCCTTCAG  CAGCTATCCT  TCCAGATGAA  1440
1441  GGTGCAGTGG  ATCTCCCTAG  CAGAAAACCT  CCTGCTCTGC  CAAATGGAAT  TGTATCAACA  1500
1501  GGGAATACAG  TAACACAGCT  TATTCCGCGA  GGGACAGACC  CCAGCTATGA  TTCTAGTCTG  1560
1561  AAACCAGGAA  AAATAGATCA  TCTGAGCAGT  AGTGCTCCTG  GATCCCCTCC  TGACCTGCTA  1620
1621  GAATCTGTTC  CTAAGAGTAT  TTCTGCCTTA  CCTGTGAATC  CACATTCTAT  ACCTCCAAGG  1680
1681  GGGCCAGCAG  ATCTGCCTCC  AATGCCTGTT  ACCAAACCAC  TTCAGATGGT  ACCTCGAGGT  1740
1741  TCTCAGTTAT  ATCCAGCACA  ACAGACGGAT  GTTTATTATC  AGGATCCTCG  AGGAGCAGCT  1800
1801  CCGCCATTTG  AACCAGCACC  TTATCAGCAG  GGTATGTATT  ATACTCCACC  ACCACAATGT  1860
1861  GTGTCCCGCT  TTGTCCGACC  TCCACCATCT  GCTCCTGAAC  CTGCTCCTCC  CTACTTGGAT  1920
1921  CATTATCCAC  CCTACCTCCA  AGAACGTGTT  GTAAACTCTC  AGTATGGCAC  ACAGCCACAG  1980
1981  CAGTACCCAC  CTATATACCC  ATCTCACTAT  GATGGCCGTC  GAGTGTACCC  TGCTCCGTCT  2040
2041  TACACAAGAG  AAGAGATATT  CCGAGAAAGC  CCTATACCCA  TTGAGATTCC  ACCTGCAGCA  2100
2101  GTACCATCGT  ATGTACCAGA  ATCCAGAGAA  AGATACCAAC  AGATCGAGAG  TTACTATCCA  2160
2161  GTGGCTCCTC  ATCCAACTCA  GATCAGACCT  TCGTACCTCA  GAGAACCTCC  TTATAGCCGG  2220
2221  CTTCCTCCTC  CTCCCCAGCC  TCATCCTAGT  CTAGATGAAC  TACATCGCCG  ACGAAAGGAA  2280
2281  ATAATGGCCC  AGCTAGAGGA  AAGAAAGGTT  ATCTCTCCAC  CTCCTTTTGC  ACCTTCACCA  2340
2341  ACCTTGCCTC  CTACCTTTCA  TCCGGAAGAA  TTTTTGGATG  AAGACTTGAA  GGTAGCTGGG  2400
2401  AAATACAAAG  GAAATGATTA  TAGCCAATAC  TCTCCCTGGT  CATGTGACAC  CATCGGCTCC  2460
2461  TACATTGGAA  CCAAAGATGC  AAAACCCAAA  GATGTTGTGG  CAGCAGGGAG  TGTGGAAATG  2520
2521  ATGAATGTGG  AGAGTAAAGG  AATGAGGGAC  CAGCGATTAG  ATCTTCAGAG  AAGAGCAGCA  2580
2581  GAAACCAGTG  ATGATGACCT  CATCCCATTT  GGAGACCGAC  CAACAGTGTC  TCGGTTTGGT  2640
2641  GCCATCTCAC  GAACTTCCAA  AACTATATAT  CAGGGTGCTG  GTCCAATGCA  GGCTATGGCA  2700
2701  CCTCAGGGAG  CTCCTACAAA  ATCTATTAAC  ATTTCAGATT  ATAGTCCATA  TGGAACCCAC  2760
2761  GGTGGCTGGG  GAGCTTCTCC  ATATTCACCT  CATCAAAACA  TACCTTCTCA  GGGACACTTC  2820
2821  AGTGAGAGGG  AGAGAATATC  TATGTCAGAA  GTGGCCAGTC  ATGGAAAACC  CCTTCCATCT  2880
2881  GCTGAGAGAG  AACAGCTACG  ACTAGAATTG  CAGCAATTGA  ACCATCAGAT  TAGCCAGCAG  2940
2941  ACCCAGCTAC  GTGGACTAGA  GGCTGTTAGT  AACAGGCTGG  TGTTGCAGAG  GGAGGCAAAC  3000
3001  ACCCTGGCAG  GCCAGTCACA  GCCCCCACCA  CCGCCACCTC  CAAAATGGCC  TGGGATGATC  3060
3061  TCAAGTGAGC  AGTTGAGCTT  GGAACTGCAC  CAGGTGGAAA  GGGAAATCGG  GAAGAGAACA  3120
3121  CGGGAACTGA  GTATGGAAAA  CCAGTGTTCT  CTGGACATGA  AAAGCAAACT  GAATACAAGT  3180
3181  AAACAAGCAG  AAAATGGACA  ACCAGAACCA  CAAAACAAGG  TTCCGGCTGA  GGACCTTACA  3240
3241  TTGACATTCA  GTGATGTACC  AAATGGATCA  GCCTTGACAC  AAGAGAATAT  CAGCCTCCTA  3300
3301  TCAAACAAGA  CCAGCTCTCT  GAACCTGTCA  GAGGACCCTG  AGGGAGGAGG  GGATAATAAT  3360
3361  GACTCCCAGA  GATCAGGAGT  TACTCCCAGT  TCTGCTCCTT  AA  3402

▼ KEYWORD

ID
Family
3D-structure
Alternative splicing
Complete proteome
Cytoplasm
Metal-binding
Phosphoprotein
Reference proteome
Repeat
RNA-binding
Transferase
Zinc
Zinc-finger

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasmic stress granule
Cellular Component
P-body
Molecular Function
Double-stranded RNA binding
Molecular Function
MiRNA binding
Molecular Function
MRNA 3'-UTR binding
Molecular Function
RNA binding
Molecular Function
RNA stem-loop binding
Molecular Function
Ubiquitin protein ligase activity
Molecular Function
Ubiquitin-protein transferase activity
Molecular Function
Zinc ion binding
Biological Process
3'-UTR-mediated mRNA destabilization
Biological Process
B cell homeostasis
Biological Process
Cellular response to interleukin-1
Biological Process
Cytoplasmic mRNA processing body assembly
Biological Process
Lymph node development
Biological Process
Negative regulation of activated T cell proliferation
Biological Process
Negative regulation of B cell proliferation
Biological Process
Negative regulation of germinal center formation
Biological Process
Negative regulation of T-helper 17 cell differentiation
Biological Process
Negative regulation of T-helper cell differentiation
Biological Process
Nuclear-transcribed mRNA catabolic process
Biological Process
Nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
Biological Process
Positive regulation of mRNA catabolic process
Biological Process
Positive regulation of NIK/NF-kappaB signaling
Biological Process
Posttranscriptional regulation of gene expression
Biological Process
Protein polyubiquitination
Biological Process
Regulation of germinal center formation
Biological Process
Regulation of miRNA metabolic process
Biological Process
Regulation of mRNA stability
Biological Process
Regulation of T cell receptor signaling pathway
Biological Process
Spleen development
Biological Process
T cell homeostasis
Biological Process
T cell proliferation
Biological Process
T cell receptor signaling pathway
Biological Process
T follicular helper cell differentiation

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Pap-4677Pan paniscus99.820.02083
LLPS-Pat-4064Pan troglodytes99.820.02083
LLPS-Nol-4175Nomascus leucogenys99.380.02078
LLPS-Man-3592Macaca nemestrina99.290.02076
LLPS-Paa-3520Papio anubis99.210.02075
LLPS-Mal-2702Mandrillus leucophaeus99.210.02073
LLPS-Cea-3321Cercocebus atys99.210.02072
LLPS-Rhb-4575Rhinopithecus bieti99.210.02072
LLPS-Mam-4182Macaca mulatta99.120.02074
LLPS-Chs-3945Chlorocebus sabaeus99.080.02013
LLPS-Aon-4130Aotus nancymaae98.940.02066
LLPS-Caj-3887Callithrix jacchus98.590.02058
LLPS-Maf-3166Macaca fascicularis97.90.02041
LLPS-Sus-2243Sus scrofa97.530.02015
LLPS-Loa-4384Loxodonta africana97.530.01975
LLPS-Fec-4358Felis catus97.350.02019
LLPS-Aim-3411Ailuropoda melanoleuca96.910.02001
LLPS-Urm-3128Ursus maritimus96.830.01999
LLPS-Ova-4359Ovis aries96.490.01976
LLPS-Mup-3081Mustela putorius furo96.290.01995
LLPS-Ict-0682Ictidomys tridecemlineatus96.210.01962
LLPS-Orc-3300Oryctolagus cuniculus96.150.01949
LLPS-Poa-1511Pongo abelii96.030.01980
LLPS-Bot-1679Bos taurus95.850.01956
LLPS-Dio-0836Dipodomys ordii95.760.01947
LLPS-Eqc-4701Equus caballus95.760.01947
LLPS-Myl-0473Myotis lucifugus95.590.01968
LLPS-Cas-3148Carlito syrichta95.060.01967
LLPS-Fud-0823Fukomys damarensis94.970.01976
LLPS-Mea-2062Mesocricetus auratus93.750.01714
LLPS-Mum-4392Mus musculus93.20.01954
LLPS-Gog-2359Gorilla gorilla92.880.01880
LLPS-Mod-2756Monodelphis domestica92.240.01885
LLPS-Sah-0175Sarcophilus harrisii91.450.01890
LLPS-Ora-1511Ornithorhynchus anatinus91.440.01883
LLPS-Cap-4341Cavia porcellus91.180.01907
LLPS-Otg-3874Otolemur garnettii90.220.01821
LLPS-Pes-3810Pelodiscus sinensis88.870.01835
LLPS-Anp-2772Anas platyrhynchos87.970.01833
LLPS-Tag-2177Taeniopygia guttata87.810.01827
LLPS-Gaga-2547Gallus gallus87.030.01797
LLPS-Fia-3347Ficedula albicollis86.810.01813
LLPS-Orl-0538Oryzias latipes79.190.0 909
LLPS-Meg-1209Meleagris gallopavo78.734e-94 306
LLPS-Dar-3673Danio rerio76.920.0 916
LLPS-Leo-1533Lepisosteus oculatus68.330.01344
LLPS-Icp-4047Ictalurus punctatus65.330.0 698
LLPS-Gaa-1718Gasterosteus aculeatus64.230.01184
LLPS-Asm-3453Astyanax mexicanus63.20.01220
LLPS-Orn-3649Oreochromis niloticus63.170.01195
LLPS-Scm-4013Scophthalmus maximus62.250.01160
LLPS-Pof-3829Poecilia formosa61.990.01149
LLPS-Ten-0490Tetraodon nigroviridis61.90.01080
LLPS-Xim-3713Xiphophorus maculatus61.580.01155
LLPS-Scf-3390Scleropages formosus57.590.01006
LLPS-Cii-0936Ciona intestinalis53.274e-61 213
LLPS-Ran-3263Rattus norvegicus51.70.0 932