LLPS-Hos-1975
ARHGEF2

▼ OVERVIEW

Status: Reviewed
Protein Name: Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40
Gene Name: ARHGEF2, KIAA0651, LFP40
Ensembl Gene: ENSG00000116584.17
Ensembl Protein: ENSP00000476916.1
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: Client
PDB: 5EFX (A) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Centrosome/Spindle pole body
"...MiCroKiTS 4.0: a database of midbody, centrosome, kinetochore, telomere and spindle."
N/A17488622
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Human cortex23071613

▼ FUNCTION

Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (By similarity).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MLSKSVPVSG  INCLLDRSDT  DGNVSQSSAI  DLRKRCSQLE  GHSGTRVGSS  LRQTFSFLSG  60
61    MTGKAKTREK  EKMKEAKDAR  YTNGHLFTTI  SVSGMTMCYA  CNKSITAKEA  LICPTCNVTI  120
121   HNRCKDTLAN  CTKVKQKQQK  AALLKNNTAL  QSVSLRSKTT  IRERPSSAIY  PSDSFRQSLL  180
181   GSRRGRSSLS  LAKSVSTTNI  AGHFNDESPL  GLRRILSQST  DSLNMRNRTL  SVESLIDEAE  240
241   VIYSELMSDF  EMDEKDFAAD  SWSLAVDSSF  LQQHKKEVMK  QQDVIYELIQ  TELHHVRTLK  300
301   IMTRLFRTGM  LEELHLEPGV  VQGLFPCVDE  LSDIHTRFLS  QLLERRRQAL  CPGSTRNFVI  360
361   HRLGDLLISQ  FSGPSAEQMC  KTYSEFCSRH  SKALKLYKEL  YARDKRFQQF  IRKVTRPAVL  420
421   KRHGVQECIL  LVTQRITKYP  LLISRILQHS  HGIEEERQDL  TTALGLVKEL  LSNVDEGIYQ  480
481   LEKGARLQEI  YNRMDPRAQT  PVPGKGPFGR  EELLRRKLIH  DGCLLWKTAT  GRFKDVLVLL  540
541   MTDVLVFLQE  KDQKYIFPTL  DKPSVVSLQN  LIVRDIANQE  KGMFLISAAP  PEMYEVHTAS  600
601   RDDRSTWIRV  IQQSVRTCPS  REDFPLIETE  DEAYLRRIKM  ELQQKDRALV  ELLREKVGLF  660
661   AEMTHFQAEE  DGGSGMALPT  LPRGLFRSES  LESPRGERLL  QDAIREVEGL  KDLLVGPGVE  720
721   LLLTPREPAL  PLEPDSGGNT  SPGVTANGEA  RTFNGSIELC  RADSDSSQRD  RNGNQLRSPQ  780
781   EEALQRLVNL  YGLLHGLQAA  VAQQDTLMEA  RFPEGPERRE  KLCRANSRDG  EAGRAGAAPV  840
841   APEKQATELA  LLQRQHALLQ  EELRRCRRLG  EERATEAGSL  EARLRESEQA  RALLEREAEE  900
901   ARRQLAALGQ  TEPLPAEAPW  ARRPVDPRRR  SLPAGDALYL  SFNPPQPSRG  TDRLDLPVTT  960
961   RSVHRNFEDR  ERQELGSPEE  RLQDSSDPDT  GSEEEGSSRL  SPPHSPRDFT  RMQDIPEETE  1020
1021  SRDGEAVASE  S  1031
Nucleotide CDS Sequence (FASTA)
1     ATGTCTCGGA  TCGAATCCCT  CACGCGGGCG  CGGATCGACC  GGAGCAGAGA  GCTGGCGAGC  60
61    AAGACCCGGG  AAAAGGAGAA  GATGAAGGAA  GCCAAGGATG  CCCGCTATAC  CAATGGGCAC  120
121   CTCTTCACCA  CCATTTCAGT  TTCAGGCATG  ACCATGTGCT  ATGCCTGTAA  CAAGAGCATC  180
181   ACAGCCAAGG  AAGCCCTCAT  CTGCCCAACC  TGCAATGTGA  CTATCCACAA  CCGCTGTAAA  240
241   GACACCCTCG  CCAACTGTAC  CAAGGTCAAG  CAGAAGCAAC  AGAAAGCGGC  CCTGCTGAAG  300
301   AACAACACCG  CCTTGCAGTC  CGTTTCTCTT  CGAAGTAAGA  CAACCATCCG  GGAGCGGCCA  360
361   AGCTCGGCCA  TCTACCCCTC  CGACAGCTTC  CGGCAGTCCC  TCCTGGGCTC  CCGCCGTGGC  420
421   CGCTCCTCCT  TGTCTTTAGC  CAAGAGTGTT  TCTACCACCA  ACATTGCTGG  ACATTTCAAT  480
481   GATGAGTCTC  CCCTGGGGCT  GCGCCGGATC  CTCTCACAGT  CCACAGACTC  CCTCAACATG  540
541   CGGAACCGAA  CCCTATCCGT  GGAATCCCTC  ATTGACGAAG  AGGTAATCTA  CAGTGAGCTG  600
601   ATGAGTGACT  TTGAGATGGA  TGAGAAGGAC  TTTGCAGCTG  ACTCTTGGAG  TCTTGCTGTG  660
661   GACAGCAGCT  TCCTGCAGCA  GCATAAAAAG  GAGGTGATGA  AGCAGCAAGA  TGTCATCTAT  720
721   GAGCTAATCC  AGACAGAGCT  GCACCATGTG  AGGACACTGA  AGATCATGAC  CCGCCTCTTC  780
781   CGCACGGGGA  TGCTGGAAGA  GCTACACTTG  GAGCCAGGAG  TGGTCCAGGG  CCTGTTCCCC  840
841   TGCGTGGACG  AGCTCAGTGA  CATCCATACA  CGCTTCCTCA  GCCAGCTATT  AGAACGCCGA  900
901   CGCCAGGCCC  TGTGCCCTGG  CAGCACCCGG  AACTTTGTCA  TCCATCGCTT  GGGTGATCTG  960
961   CTCATCAGCC  AGTTCTCAGG  TCCTAGTGCG  GAGCAGATGT  GTAAGACCTA  CTCGGAGTTC  1020
1021  TGCAGCCGCC  ACAGCAAGGC  CTTAAAGCTC  TATAAGGAGC  TGTACGCCCG  AGACAAACGC  1080
1081  TTCCAGCAAT  TCATCCGGAA  AGTGACCCGC  CCCGCCGTGC  TCAAGCGGCA  CGGGGTACAG  1140
1141  GAGTGCATCC  TGCTGGTGAC  TCAGCGCATC  ACCAAGTACC  CGTTACTCAT  CAGCCGCATC  1200
1201  CTGCAGCATT  CCCACGGGAT  CGAGGAGGAG  CGCCAGGACC  TGACCACAGC  ACTGGGGCTA  1260
1261  GTGAAGGAGC  TGCTGTCCAA  TGTGGACGAG  GGTATTTATC  AGCTGGAGAA  AGGGGCCCGT  1320
1321  CTGCAGGAGA  TCTACAACCG  CATGGACCCT  CGGGCCCAAA  CCCCAGTGCC  TGGCAAGGGC  1380
1381  CCCTTTGGCC  GAGAGGAACT  TCTGAGGCGC  AAACTCATCC  ACGATGGCTG  CCTGCTCTGG  1440
1441  AAGACAGCGA  CGGGGCGCTT  CAAAGATGTG  CTAGTGCTGC  TGATGACAGA  TGTACTGGTG  1500
1501  TTTCTCCAGG  AAAAGGACCA  GAAGTACATC  TTTCCTACCC  TGGACAAGCC  TTCAGTGGTA  1560
1561  TCGCTGCAGA  ATCTAATCGT  ACGAGACATT  GCCAACCAGG  AGAAAGGGAT  GTTTCTGATC  1620
1621  AGCGCAGCCC  CACCTGAGAT  GTACGAGGTG  CACACAGCAT  CCCGGGATGA  CCGGAGCACC  1680
1681  TGGATCCGGG  TCATTCAGCA  GAGCGTGCGC  ACATGCCCAT  CCAGGGAGGA  CTTCCCCCTG  1740
1741  ATTGAGACAG  AGGATGAGGC  TTACCTGCGG  CGAATTAAGA  TGGAGTTGCA  GCAGAAGGAC  1800
1801  CGGGCACTGG  TGGAGCTGCT  GCGAGAGAAG  GTCGGGCTGT  TTGCTGAGAT  GACCCATTTC  1860
1861  CAGGCCGAAG  AGGATGGTGG  CAGTGGGATG  GCCCTGCCCA  CCCTGCCCAG  GGGCCTTTTC  1920
1921  CGCTCTGAGT  CCCTTGAGTC  CCCTCGTGGC  GAGCGGCTGC  TGCAGGATGC  CATCCGTGAG  1980
1981  GTGGAGGGTC  TGAAAGACCT  GCTGGTGGGG  CCAGGAGTGG  AACTGCTCTT  GACACCCCGA  2040
2041  GAGCCAGCCC  TGCCCTTGGA  ACCAGACAGC  GGTGGTAACA  CGAGTCCTGG  GGTCACTGCC  2100
2101  AATGGTGAGG  CCAGAACCTT  CAATGGCTCC  ATTGAACTCT  GCAGAGCTGA  CTCAGACTCT  2160
2161  AGCCAGAGGG  ATCGAAATGG  AAATCAGCTG  AGATCACCGC  AAGAGGAGGC  GTTACAGCGA  2220
2221  TTGGTCAATC  TCTATGGACT  TCTACATGGC  CTACAGGCAG  CTGTGGCCCA  GCAGGACACT  2280
2281  CTGATGGAAG  CCCGGTTCCC  TGAGGGCCCT  GAGCGGCGGG  AGAAGCTGTG  CCGAGCCAAC  2340
2341  TCTCGGGATG  GGGAGGCTGG  CAGGGCTGGG  GCTGCCCCTG  TGGCCCCTGA  AAAGCAGGCC  2400
2401  ACGGAACTGG  CATTACTGCA  GCGGCAACAT  GCGCTGCTGC  AGGAGGAGCT  ACGGCGCTGC  2460
2461  CGGCGGCTAG  GTGAAGAACG  GGCAACCGAA  GCTGGCAGCC  TGGAGGCCCG  GCTCCGGGAG  2520
2521  AGTGAGCAGG  CCCGGGCACT  GCTGGAGCGT  GAGGCCGAAG  AGGCTCGAAG  GCAGCTGGCC  2580
2581  GCCCTGGGCC  AGACCGAGCC  ACTCCCAGCT  GAGGCCCCCT  GGGCCCGCAG  ACCTGTGGAT  2640
2641  CCTCGGCGGC  GCAGCCTCCC  CGCAGGCGAT  GCCCTGTACT  TGAGTTTCAA  CCCCCCACAG  2700
2701  CCCAGCCGAG  GCACTGACCG  CCTGGATCTA  CCTGTCACTA  CTCGCTCTGT  CCATCGAAAC  2760
2761  TTTGAGGACC  GAGAGAGGCA  GGAACTGGGG  AGCCCCGAAG  AGCGGCTGCA  AGACAGCAGT  2820
2821  GACCCTGACA  CTGGCAGCGA  GGAGGAAGGT  AGCAGCCGTC  TGTCTCCGCC  CCACAGTCCA  2880
2881  CGAGACTTTA  CCAGAATGCA  GGACATCCCG  GAGGAGACGG  AGAGCCGCGA  CGGGGAGGCT  2940
2941  GTAGCCTCCG  AGAGCTAA  2958

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Alternative splicing
Cell cycle
Cell division
Cell junction
Cell membrane
Cell projection
Coiled coil
Complete proteome
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
Developmental protein
Differentiation
Golgi apparatus
Guanine-nucleotide releasing factor
Immunity
Innate immunity
Membrane
Mental retardation
Metal-binding
Microtubule
Mitosis
Neurogenesis
Phosphoprotein
Reference proteome
Tight junction
Zinc
Zinc-finger

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Bicellular tight junction
Cellular Component
Cytoplasm
Cellular Component
Cytoplasmic vesicle
Cellular Component
Cytoskeleton
Cellular Component
Cytosol
Cellular Component
Dendritic shaft
Cellular Component
Focal adhesion
Cellular Component
Glutamatergic synapse
Cellular Component
Golgi apparatus
Cellular Component
Microtubule
Cellular Component
Neuronal cell body
Cellular Component
Podosome
Cellular Component
Postsynaptic density, intracellular component
Cellular Component
Protein-containing complex
Cellular Component
Ruffle membrane
Cellular Component
Spindle
Cellular Component
Vesicle
Molecular Function
Microtubule binding
Molecular Function
Rac GTPase binding
Molecular Function
Rac guanyl-nucleotide exchange factor activity
Molecular Function
Rho GTPase binding
Molecular Function
Rho guanyl-nucleotide exchange factor activity
Molecular Function
Transcription factor binding
Molecular Function
Zinc ion binding
Biological Process
Actin filament organization
Biological Process
Asymmetric neuroblast division
Biological Process
Cell morphogenesis
Biological Process
Cellular hyperosmotic response
Biological Process
Cellular response to muramyl dipeptide
Biological Process
Cellular response to tumor necrosis factor
Biological Process
Establishment of mitotic spindle orientation
Biological Process
G protein-coupled receptor signaling pathway
Biological Process
Innate immune response
Biological Process
Intracellular protein transport
Biological Process
Intracellular signal transduction
Biological Process
Negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
Biological Process
Negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress
Biological Process
Negative regulation of microtubule depolymerization
Biological Process
Negative regulation of necroptotic process
Biological Process
Negative regulation of neurogenesis
Biological Process
Negative regulation of podosome assembly
Biological Process
Positive regulation of apoptotic process
Biological Process
Positive regulation of interleukin-6 production
Biological Process
Positive regulation of neuron differentiation
Biological Process
Positive regulation of neuron migration
Biological Process
Positive regulation of NF-kappaB transcription factor activity
Biological Process
Positive regulation of peptidyl-tyrosine phosphorylation
Biological Process
Positive regulation of transcription by RNA polymerase II
Biological Process
Positive regulation of tumor necrosis factor production
Biological Process
Regulation of cell population proliferation
Biological Process
Regulation of Rho protein signal transduction
Biological Process
Regulation of small GTPase mediated signal transduction

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Gog-3253Gorilla gorilla99.220.01882
LLPS-Pat-1321Pan troglodytes98.930.01875
LLPS-Poa-4246Pongo abelii98.350.01739
LLPS-Chs-4770Chlorocebus sabaeus98.140.01739
LLPS-Paa-3799Papio anubis97.870.01853
LLPS-Maf-2862Macaca fascicularis97.770.01850
LLPS-Mam-4180Macaca mulatta97.580.01837
LLPS-Nol-3473Nomascus leucogenys97.570.01845
LLPS-Cea-2380Cercocebus atys97.190.01831
LLPS-Caj-2381Callithrix jacchus96.410.01826
LLPS-Aon-3271Aotus nancymaae96.320.01813
LLPS-Pap-2233Pan paniscus95.640.01791
LLPS-Orc-3060Oryctolagus cuniculus95.250.01683
LLPS-Ict-3843Ictidomys tridecemlineatus95.150.01416
LLPS-Cap-1453Cavia porcellus94.830.01664
LLPS-Otg-0944Otolemur garnettii94.320.01632
LLPS-Dio-2842Dipodomys ordii94.20.01644
LLPS-Sus-1624Sus scrofa93.90.01757
LLPS-Rhb-4376Rhinopithecus bieti93.890.01734
LLPS-Eqc-4023Equus caballus93.80.01758
LLPS-Man-1291Macaca nemestrina93.790.01734
LLPS-Loa-4528Loxodonta africana93.50.01651
LLPS-Myl-2674Myotis lucifugus93.490.01639
LLPS-Cas-0100Carlito syrichta93.310.01712
LLPS-Mal-3613Mandrillus leucophaeus93.310.01751
LLPS-Ova-1718Ovis aries92.530.01518
LLPS-Fec-4332Felis catus91.660.01718
LLPS-Mea-3281Mesocricetus auratus91.542e-117 369
LLPS-Caf-3050Canis familiaris91.380.01701
LLPS-Bot-4054Bos taurus91.240.01617
LLPS-Aim-1815Ailuropoda melanoleuca91.160.01255
LLPS-Mod-0272Monodelphis domestica88.790.01531
LLPS-Mum-3279Mus musculus88.620.01549
LLPS-Ran-4461Rattus norvegicus87.690.01620
LLPS-Sah-2154Sarcophilus harrisii87.080.01590
LLPS-Urm-2636Ursus maritimus82.390.01061
LLPS-Anc-1789Anolis carolinensis75.079e-177 528
LLPS-Lac-3009Latimeria chalumnae65.360.0 942
LLPS-Asm-2987Astyanax mexicanus61.720.0 852
LLPS-Gaa-1587Gasterosteus aculeatus61.30.0 726
LLPS-Scm-1892Scophthalmus maximus61.00.0 746
LLPS-Icp-2399Ictalurus punctatus60.930.0 851
LLPS-Dar-0470Danio rerio58.750.0 813
LLPS-Scf-3973Scleropages formosus57.580.0 842
LLPS-Ten-3757Tetraodon nigroviridis57.050.0 742
LLPS-Orn-2264Oreochromis niloticus56.930.0 760
LLPS-Xim-1534Xiphophorus maculatus56.580.0 722
LLPS-Pof-3470Poecilia formosa56.180.0 762
LLPS-Tar-3781Takifugu rubripes55.880.0 736
LLPS-Orl-3621Oryzias latipes55.580.0 734
LLPS-Ora-2211Ornithorhynchus anatinus39.663e-77 274
LLPS-Tut-1103Tursiops truncatus27.921e-0966.6
LLPS-Xet-0407Xenopus tropicalis27.761e-32 142
LLPS-Pes-3668Pelodiscus sinensis27.762e-33 144
LLPS-Tag-0303Taeniopygia guttata27.522e-33 144
LLPS-Leo-2812Lepisosteus oculatus27.511e-32 142
LLPS-Fud-4079Fukomys damarensis27.496e-1377.8
LLPS-Fia-3515Ficedula albicollis27.324e-33 143
LLPS-Gaga-1141Gallus gallus27.274e-33 143
LLPS-Meg-0922Meleagris gallopavo27.275e-33 143
LLPS-Anp-0608Anas platyrhynchos26.783e-32 140
LLPS-Cii-0843Ciona intestinalis26.674e-0965.1
LLPS-Mup-3592Mustela putorius furo26.643e-34 147
LLPS-Cae-1818Caenorhabditis elegans25.717e-21 103
LLPS-Usm-1517Ustilago maydis24.015e-1068.2
LLPS-Mel-0205Melampsora laricipopulina23.66e-1171.2