DrLLPS - Data resource of LLPS

Overview
Property
Classification
Function
Cross Reference
Sequence
GO
Annotation
Orthology
TOP

LLPS-Hos-0500
UBE2I

▼ OVERVIEW


Status:	Reviewed
Protein Name:	SUMO-conjugating enzyme UBC9; RING-type E3 SUMO transferase UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
Gene Name:	UBE2I, UBC9, UBCE9
Ensembl Gene:	ENSG00000103275.19
Ensembl Protein:	ENSP00000324897.5
Organism:	Homo sapiens
Taxa ID:	9606
LLPS Type:	LLPS regulator

PDB: 1Z5S (A, B, C, D) More

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Description	Tissue/Cell	PMIDs
PML nuclear body	"...Ubc9 regulates the interaction between CACUL1 and PML."	HeLa cells	27889610
PML nuclear body	"...More important, the formation of SUMO5-induced NBs was impaired by coexpression of Ubc9, a dominant negative mutant of Ubc9, or SENP1, the SUMO de-conjugating protease."	293T cells	27211601
PML nuclear body	"...First, UBC9 does not localize readily or accumulates to PML-NBs under basal conditions, but rather requires a stimulus. Second, the integrity of Lys-49 is important for UBC9 colocalization to PML-NBs."	U2OS cells	29773808
Stress granule	"...Fraction of SGs colocalizing with Ubc9TS‐mCherry or Ubc9WT‐mCherry in cells expressing G3BP2‐GFP."	HeLa cells	28377462
Others	"...PMLOs are different in size, shape, and composition, and almost invariantly contain intrinsically disordered proteins, which indicates that the formation of these phaseseparated droplets is crucially dependent on intrinsic disorder."	N/A	27838525
Cajal body, Nuclear pore complex, Nuclear speckle, Nucleolus, P-body	Predicted from orthologs	N/A	(View)

Organism	DrLLPS ID	UniProt ID	LLPS type	Condensate	Identity	E-value	Score
Caenorhabditis elegans	LLPS-Cae-0230	Q95017	Regulator	P-body	78.10	2e-80	243
Mus musculus	LLPS-Mum-1177	P63280	Client	Cajal body, Nuclear pore complex, Nuclear speckle, Nucleolus, PML nuclear body	100.00	2e-98	288
Gallus gallus	LLPS-Gaga-0287	P63283	Client	Nucleolus	100.00	2e-98	288

▼ FUNCTION

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	ENST00000325437.9	ENSP00000324897.5
Ensembl	ENST00000406620.5	ENSP00000384568.1
Ensembl	ENST00000397514.7	ENSP00000380649.3
Ensembl	ENST00000397515.6	ENSP00000380650.2
Ensembl	ENST00000562470.2	ENSP00000490882.1
Ensembl	ENST00000567383.5	ENSP00000456188.1
Ensembl	ENST00000567074.5	ENSP00000455893.1
Ensembl	ENST00000402301.5	ENSP00000384361.1
Ensembl	ENST00000566775.1	ENSP00000455252.1
Ensembl	ENST00000403747.6	ENSP00000385009.2
Ensembl	ENST00000566587.5	ENSP00000457064.1
Ensembl	ENST00000355803.8	ENSP00000348056.4
UniProt		P63279, UBC9_HUMAN, D3DU69, P50550, Q15698, Q59GX1, Q86VB3
GeneBank	X96427, U66818, CH471112, U31882, U38785, AJ002385, BT006932, U31933, AL031714, U29092, BC004429, BC000427, BC051289, U45328, AE006466, D45050, U66867, AB208988	BAD92225.1, AAC50603.1, AAC50716.1, AAP35578.1, AAC50715.1, CAA05359.1, EAW85677.1, EAW85678.1, EAW85676.1, AAH00427.1, AAC51361.1, CAA65287.1, EAW85673.1, AAH51289.3, AAK61274.1, AAA86662.1, AAB09410.1, BAA08091.1, EAW85679.1, AAH04429.1, AAB02181.1
RefSeq	NM_003345.4, NM_194259.2, NM_194260.2, NM_194261.2, XM_017023640.1	NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1, XP_016879129.1
Entrez	7329

▼ SEQUENCE

Protein Sequence (FASTA)
1 MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL 60 61 RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL 120 121 NEPNIQDPAQ AEAYTIYWLV AALAPLVAAP PRPSQGRLAG REWRTQAHPT DSQGPRLCGE 180 181 GRGA 184
Nucleotide CDS Sequence (FASTA)
1 ATGTCGGGGA TCGCCCTCAG CAGACTCGCC CAGGAGAGGA AAGCATGGAG GAAAGACCAC 60 61 CCATTTGGTT TCGTGGCTGT CCCAACAAAA AATCCCGATG GCACGATGAA CCTCATGAAC 120 121 TGGGAGTGCG CCATTCCAGG AAAGAAAGGG ACTCCGTGGG AAGGAGGCTT GTTTAAACTA 180 181 CGGATGCTTT TCAAAGATGA TTATCCATCT TCGCCACCAA AATGTAAATT CGAACCACCA 240 241 TTATTTCACC CGAATGTGTA CCCTTCGGGG ACAGTGTGCC TGTCCATCTT AGAGGAGGAC 300 301 AAGGACTGGA GGCCAGCCAT CACAATCAAA CAGATCCTAT TAGGAATACA GGAACTTCTA 360 361 AATGAACCAA ATATCCAAGA CCCAGCTCAA GCAGAGGCCT ACACGATTTA CTGCCAAAAC 420 421 AGAGTGGAGT ACGAGAAAAG GGTCCGAGCA CAAGCCAAGA AGTTTGCGCC CTCATAA 477

▼ KEYWORD

ID	Family
KW-0002	3D-structure
KW-0007	Acetylation
KW-0067	ATP-binding
KW-0131	Cell cycle
KW-0132	Cell division
KW-0159	Chromosome partition
KW-0181	Complete proteome
KW-0963	Cytoplasm
KW-0945	Host-virus interaction
KW-1017	Isopeptide bond
KW-0498	Mitosis
KW-0547	Nucleotide-binding
KW-0539	Nucleus
KW-0597	Phosphoprotein
KW-1185	Reference proteome
KW-0808	Transferase
KW-0832	Ubl conjugation
KW-0833	Ubl conjugation pathway

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0005737	Cellular Component	Cytoplasm
GO:0005829	Cellular Component	Cytosol
GO:0030425	Cellular Component	Dendrite
GO:0001650	Cellular Component	Fibrillar center
GO:0005635	Cellular Component	Nuclear envelope
GO:0005654	Cellular Component	Nucleoplasm
GO:0005634	Cellular Component	Nucleus
GO:0016605	Cellular Component	PML body
GO:1990356	Cellular Component	Sumoylated E2 ligase complex
GO:0045202	Cellular Component	Synapse
GO:0000795	Cellular Component	Synaptonemal complex
GO:1990234	Cellular Component	Transferase complex
GO:0005524	Molecular Function	ATP binding
GO:0043425	Molecular Function	BHLH transcription factor binding
GO:0019899	Molecular Function	Enzyme binding
GO:0043398	Molecular Function	HLH domain binding
GO:0008022	Molecular Function	Protein C-terminus binding
GO:0071535	Molecular Function	RING-like zinc finger domain binding
GO:0003723	Molecular Function	RNA binding
GO:0044388	Molecular Function	Small protein activating enzyme binding
GO:0061656	Molecular Function	SUMO conjugating enzyme activity
GO:0019789	Molecular Function	SUMO transferase activity
GO:0008134	Molecular Function	Transcription factor binding
GO:0004842	Molecular Function	Ubiquitin-protein transferase activity
GO:0007049	Biological Process	Cell cycle
GO:0051301	Biological Process	Cell division
GO:0006464	Biological Process	Cellular protein modification process
GO:0007059	Biological Process	Chromosome segregation
GO:0000122	Biological Process	Negative regulation of transcription by RNA polymerase II
GO:0045892	Biological Process	Negative regulation of transcription, DNA-templated
GO:0051091	Biological Process	Positive regulation of DNA-binding transcription factor activity
GO:0043123	Biological Process	Positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0033145	Biological Process	Positive regulation of intracellular steroid hormone receptor signaling pathway
GO:1903755	Biological Process	Positive regulation of SUMO transferase activity
GO:0043161	Biological Process	Proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0016925	Biological Process	Protein sumoylation
GO:0010469	Biological Process	Regulation of signaling receptor activity
GO:0006511	Biological Process	Ubiquitin-dependent protein catabolic process
GO:0016032	Biological Process	Viral process

▼ KEGG

hsa:7329

▼ ANNOTATION

Disorder
D²P²	IUPred2A

Domain
SMART	Pfam	InterPro	CDD	PROSITE
Gene3D

PTM
EPSD	PLMD	PhosphoSitePlus	dbPTM	HPRD
BioGRID	mUbiSiDa	PTMcode

Variation
dbSNP	VarCards

Mutation
TCGA	ICGC	CGAP	IntOGen	BioMuta

Interaction
HINT	InWeb_IM	RISE	TRRUST	BindingDB
RAIN	Mentha	PINA	IID	iRefIndex
MIST	DifferentialNET	RNAct

Disease
PTMD	TumorFusions	OMIM	DisGeNET	ActiveDriverDB

Drug
CTD

Physicochemical
Compute pI/Mw	AAindex

Function
PIRSF	CORUM	CellMarker	RaftProt

Proteomics
THPA	HPM

Structure
PDB

Localization
COMPARTMENTS

Expression
TCGA	ICGC	COSMIC	GEO	ArrayExpress
TISSUES	BioXpress	THPA

Element
UTRdb	circBase	circRNADb	CircNet	miRTarBase
miRWalk	miRecords	miRcode	RAID	microRNA.org
m6AVar

Methylation
TCGA	ICGC	MethyCancer

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Otg-0444	Otolemur garnettii	100.0	1e-97	286
LLPS-Pes-1844	Pelodiscus sinensis	100.0	6e-98	288
LLPS-Fia-1470	Ficedula albicollis	100.0	6e-98	288
LLPS-Orn-0018	Oreochromis niloticus	100.0	5e-98	288
LLPS-Dar-0681	Danio rerio	100.0	7e-98	287
LLPS-Meg-0569	Meleagris gallopavo	100.0	5e-98	288
LLPS-Pat-1249	Pan troglodytes	100.0	4e-98	289
LLPS-Pof-2447	Poecilia formosa	100.0	4e-98	288
LLPS-Urm-2312	Ursus maritimus	100.0	3e-37	130
LLPS-Pap-2548	Pan paniscus	100.0	5e-98	288
LLPS-Scm-0926	Scophthalmus maximus	100.0	5e-98	288
LLPS-Mod-0308	Monodelphis domestica	100.0	5e-98	288
LLPS-Fec-0507	Felis catus	100.0	5e-98	288
LLPS-Man-3364	Macaca nemestrina	100.0	5e-98	288
LLPS-Anp-0192	Anas platyrhynchos	100.0	6e-98	288
LLPS-Loa-1145	Loxodonta africana	100.0	5e-98	288
LLPS-Gaga-0287	Gallus gallus	100.0	5e-98	288
LLPS-Xet-0108	Xenopus tropicalis	100.0	9e-98	287
LLPS-Anc-1247	Anolis carolinensis	100.0	5e-98	288
LLPS-Ict-2623	Ictidomys tridecemlineatus	100.0	5e-98	288
LLPS-Tag-1291	Taeniopygia guttata	100.0	6e-98	288
LLPS-Poa-3044	Pongo abelii	100.0	5e-98	288
LLPS-Cap-1230	Cavia porcellus	100.0	5e-98	288
LLPS-Eqc-0487	Equus caballus	100.0	3e-96	294
LLPS-Mam-0561	Macaca mulatta	100.0	5e-98	288
LLPS-Gog-1730	Gorilla gorilla	100.0	5e-98	288
LLPS-Asm-0803	Astyanax mexicanus	100.0	5e-98	288
LLPS-Fud-1051	Fukomys damarensis	100.0	5e-98	288
LLPS-Caj-0525	Callithrix jacchus	100.0	4e-98	290
LLPS-Icp-1330	Ictalurus punctatus	100.0	5e-98	288
LLPS-Maf-2400	Macaca fascicularis	100.0	5e-98	288
LLPS-Mum-1177	Mus musculus	100.0	5e-98	288
LLPS-Mea-0119	Mesocricetus auratus	100.0	5e-98	288
LLPS-Ora-1644	Ornithorhynchus anatinus	100.0	2e-49	161
LLPS-Chs-1229	Chlorocebus sabaeus	100.0	5e-98	288
LLPS-Orl-1148	Oryzias latipes	100.0	5e-98	288
LLPS-Leo-1025	Lepisosteus oculatus	100.0	6e-98	288
LLPS-Sus-0668	Sus scrofa	100.0	5e-98	288
LLPS-Aon-3784	Aotus nancymaae	100.0	5e-98	288
LLPS-Ran-1666	Rattus norvegicus	100.0	5e-98	288
LLPS-Mup-1206	Mustela putorius furo	100.0	5e-98	288
LLPS-Aim-0758	Ailuropoda melanoleuca	100.0	5e-98	288
LLPS-Myl-0610	Myotis lucifugus	100.0	5e-98	288
LLPS-Ova-1162	Ovis aries	100.0	6e-97	287
LLPS-Xim-0205	Xiphophorus maculatus	100.0	4e-98	288
LLPS-Tut-0872	Tursiops truncatus	100.0	1e-58	186
LLPS-Dio-3410	Dipodomys ordii	100.0	5e-98	288
LLPS-Gaa-3564	Gasterosteus aculeatus	100.0	5e-98	288
LLPS-Scf-1071	Scleropages formosus	100.0	7e-98	287
LLPS-Bot-2126	Bos taurus	100.0	5e-98	288
LLPS-Nol-3452	Nomascus leucogenys	99.27	3e-97	286
LLPS-Caf-2422	Canis familiaris	99.11	2e-78	237
LLPS-Tar-0931	Takifugu rubripes	97.81	5e-96	283
LLPS-Lac-0083	Latimeria chalumnae	97.08	4e-96	283
LLPS-Ten-2106	Tetraodon nigroviridis	96.35	1e-94	279
LLPS-Mal-1397	Mandrillus leucophaeus	94.02	5e-112	325
LLPS-Cea-1706	Cercocebus atys	93.44	3e-111	324
LLPS-Paa-1264	Papio anubis	93.44	3e-111	324
LLPS-Rhb-3535	Rhinopithecus bieti	92.9	2e-111	324
LLPS-Orc-0697	Oryctolagus cuniculus	91.24	5e-85	254
LLPS-Sah-2514	Sarcophilus harrisii	89.21	3e-85	255
LLPS-Cas-1543	Carlito syrichta	87.73	2e-97	286
LLPS-Drm-0550	Drosophila melanogaster	87.59	4e-88	262
LLPS-Cii-0448	Ciona intestinalis	85.4	4e-86	257
LLPS-Cis-1597	Ciona savignyi	83.94	8e-85	254
LLPS-Cae-0230	Caenorhabditis elegans	78.1	2e-80	243
LLPS-Usm-0023	Ustilago maydis	72.99	4e-72	222
LLPS-Spr-0971	Sporisorium reilianum	72.99	1e-72	223
LLPS-Asni-0115	Aspergillus niger	69.6	2e-60	192
LLPS-Phv-0536	Phaseolus vulgaris	69.34	4e-65	204
LLPS-Mel-0577	Melampsora laricipopulina	69.34	2e-68	212
LLPS-Glm-0242	Glycine max	69.34	4e-65	204
LLPS-Met-1266	Medicago truncatula	69.34	6e-67	208
LLPS-Scj-0422	Schizosaccharomyces japonicus	69.34	1e-67	210
LLPS-Vir-1593	Vigna radiata	69.0	4e-44	149
LLPS-Prp-0500	Prunus persica	68.89	8e-64	201
LLPS-Sem-0186	Selaginella moellendorffii	68.89	4e-65	204
LLPS-Asfu-0278	Aspergillus fumigatus	68.8	3e-60	192
LLPS-Brr-3007	Brassica rapa	68.38	1e-64	202
LLPS-Bro-1691	Brassica oleracea	68.38	1e-64	202
LLPS-Coc-0727	Corchorus capsularis	68.38	5e-65	204
LLPS-Brn-0055	Brassica napus	68.38	1e-64	202
LLPS-Sot-0618	Solanum tuberosum	68.38	3e-64	202
LLPS-Sob-0037	Sorghum bicolor	68.15	5e-63	199
LLPS-Mae-1308	Manihot esculenta	68.15	1e-62	198
LLPS-Pot-1656	Populus trichocarpa	68.15	3e-63	199
LLPS-Zem-2150	Zea mays	68.15	3e-63	199
LLPS-Thc-0189	Theobroma cacao	68.15	5e-62	196
LLPS-Sei-1279	Setaria italica	68.15	5e-63	199
LLPS-Lep-0397	Leersia perrieri	68.15	6e-63	200
LLPS-Scc-0516	Schizosaccharomyces cryophilus	67.88	1e-66	207
LLPS-Chc-0351	Chondrus crispus	67.88	5e-64	201
LLPS-Art-2165	Arabidopsis thaliana	67.65	2e-62	197
LLPS-Arl-1972	Arabidopsis lyrata	67.65	2e-62	197
LLPS-Orbr-1460	Oryza brachyantha	67.41	4e-62	196
LLPS-Ors-0924	Oryza sativa	67.41	6e-62	196
LLPS-Ast-0085	Aspergillus terreus	67.41	1e-63	200
LLPS-Orp-1153	Oryza punctata	67.41	4e-62	196
LLPS-Ved-1369	Verticillium dahliae	67.41	3e-64	201
LLPS-Org-1019	Oryza glaberrima	67.41	6e-62	196
LLPS-Tru-0735	Triticum urartu	67.41	4e-62	196
LLPS-Orr-0346	Oryza rufipogon	67.41	6e-62	196
LLPS-Asn-1008	Aspergillus nidulans	67.41	6e-64	201
LLPS-Orni-1293	Oryza nivara	67.41	6e-62	196
LLPS-Mua-0768	Musa acuminata	67.41	8e-63	198
LLPS-Orb-0941	Oryza barthii	67.41	6e-62	196
LLPS-Ori-0706	Oryza indica	67.41	6e-62	196
LLPS-Hov-0843	Hordeum vulgare	67.41	2e-60	193
LLPS-Cus-0690	Cucumis sativus	67.41	2e-62	197
LLPS-Tra-1879	Triticum aestivum	67.41	4e-62	196
LLPS-Brd-2375	Brachypodium distachyon	67.41	7e-62	196
LLPS-Nec-0005	Neurospora crassa	67.41	3e-63	199
LLPS-Zyt-0071	Zymoseptoria tritici	67.41	2e-63	199
LLPS-Via-0062	Vigna angularis	67.41	3e-62	196
LLPS-Gor-1634	Gossypium raimondii	67.39	6e-61	193
LLPS-Viv-1614	Vitis vinifera	66.91	5e-62	196
LLPS-Abg-1129	Absidia glauca	66.91	4e-64	201
LLPS-Nef-0835	Neosartorya fischeri	66.67	6e-64	201
LLPS-Amt-1193	Amborella trichopoda	66.67	7e-63	198
LLPS-Nia-1969	Nicotiana attenuata	66.67	9e-64	200
LLPS-Orm-0687	Oryza meridionalis	66.67	2e-61	195
LLPS-Hea-1668	Helianthus annuus	66.67	3e-62	197
LLPS-Php-0159	Physcomitrella patens	66.67	9e-62	195
LLPS-Dac-0953	Daucus carota	66.67	3e-62	197
LLPS-Orgl-0117	Oryza glumaepatula	66.67	2e-61	195
LLPS-Scp-0529	Schizosaccharomyces pombe	66.42	1e-64	202
LLPS-Dos-0328	Dothistroma septosporum	65.93	9e-62	195
LLPS-Coo-0950	Colletotrichum orbiculare	65.93	9e-63	198
LLPS-Gag-0901	Gaeumannomyces graminis	65.93	2e-62	200
LLPS-Sol-2007	Solanum lycopersicum	65.93	6e-62	196
LLPS-Asc-0550	Aspergillus clavatus	65.93	6e-63	198
LLPS-Cog-0707	Colletotrichum gloeosporioides	65.67	8e-60	190
LLPS-Trr-0328	Trichoderma reesei	65.19	2e-63	199
LLPS-Trv-0843	Trichoderma virens	65.19	3e-63	199
LLPS-Map-1121	Magnaporthe poae	65.19	3e-62	196
LLPS-Aso-0095	Aspergillus oryzae	65.19	1e-62	197
LLPS-Crn-0607	Cryptococcus neoformans	64.96	6e-66	206
LLPS-Cogr-0019	Colletotrichum graminicola	64.71	9e-60	190
LLPS-Fus-0669	Fusarium solani	64.44	4e-62	196
LLPS-Fuo-0496	Fusarium oxysporum	64.44	1e-62	197
LLPS-Fuv-0413	Fusarium verticillioides	64.44	1e-62	197
LLPS-Asf-0141	Aspergillus flavus	64.44	3e-62	196
LLPS-Blg-0958	Blumeria graminis	64.44	2e-62	197
LLPS-Chr-0645	Chlamydomonas reinhardtii	64.23	2e-61	194
LLPS-Miv-0388	Microbotryum violaceum	63.97	1e-58	187
LLPS-Phn-0514	Phaeosphaeria nodorum	63.77	2e-61	194
LLPS-Cym-0093	Cyanidioschyzon merolae	63.5	5e-59	189
LLPS-Lem-0259	Leptosphaeria maculans	63.43	9e-62	195
LLPS-Pytr-0956	Pyrenophora triticirepentis	62.96	2e-61	194
LLPS-Mao-0610	Magnaporthe oryzae	62.22	2e-61	194
LLPS-Pyt-0458	Pyrenophora teres	62.22	4e-61	194
LLPS-Beb-1012	Beauveria bassiana	61.48	4e-59	188
LLPS-Kop-0911	Komagataella pastoris	60.58	2e-56	182
LLPS-Osl-0894	Ostreococcus lucimarinus	59.42	1e-55	180
LLPS-Asg-0038	Ashbya gossypii	59.12	8e-56	180
LLPS-Gas-0711	Galdieria sulphuraria	58.52	5e-54	175
LLPS-Sac-0935	Saccharomyces cerevisiae	58.39	2e-55	179
LLPS-Tum-0257	Tuber melanosporum	56.93	4e-48	160
LLPS-Yal-0626	Yarrowia lipolytica	54.62	3e-52	171

LLPS-Hos-0500UBE2I

Protein Sequence

Nucleotide Sequence

LLPS-Hos-0500
UBE2I