LLPS-Hos-0494
EEF2

▼ OVERVIEW

Status: Reviewed
Protein Name: Elongation factor 2
Gene Name: EEF2, EF2
Ensembl Gene: ENSG00000167658.15
Ensembl Protein: ENSP00000307940.5
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Nucleolus
"...The data set from all our nucleolar mass spectrometry analyses defines an updated group of 692 proteins that reproducibly copurify with human nucleoli."
HeLa cells15635413
Nucleolus
"...Thus, we analyzed the orthologous groups of the remaining 2316 identified proteins to the human nucleolar proteins. We detected 361 orthologues in NoPDB from the 2316 A. thaliana proteins."
N/A26980300
Stress granule
"...EEF2 and Ras-GAP SH3 domain-binding protein modulate stress granule assembly during HIV-1 infection."
HEK293T cells25229650
Stress granule
"...Using an RNA-mediated interference-based screen, we identify 101 human genes required for SG assembly, 39 genes required for PB assembly, and 31 genes required for coordinate SG and PB assembly."
U2OS cells18794846
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Human cortex23071613

▼ FUNCTION

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MVNFTVDQIR  AIMDKKANIR  NMSVIAHVDH  GKSTLTDSLV  CKAGIIASAR  AGETRFTDTR  60
61    KDEQERCITI  KSTAISLFYE  LSENDLNFIK  QSKDGAGFLI  NLIDSPGHVD  FSSEVTAALR  120
121   VTDGALVVVD  CVSGVCVQTE  TVLRQAIAER  IKPVLMMNKM  DRALLELQLE  PEELYQTFQR  180
181   IVENVNVIIS  TYGEGESGPM  GNIMIDPVLG  TVGFGSGLHG  WAFTLKQFAE  MYVAKFAAKG  240
241   EGQLGPAERA  KKVEDMMKKL  WGDRYFDPAN  GKFSKSATSP  EGKKLPRTFC  QLILDPIFKV  300
301   FDAIMNFKKE  ETAKLIEKLD  IKLDSEDKDK  EGKPLLKAVM  RRWLPAGDAL  LQMITIHLPS  360
361   PVTAQKYRCE  LLYEGPPDDE  AAMGIKSCDP  KGPLMMYISK  MVPTSDKGRF  YAFGRVFSGL  420
421   VSTGLKVRIM  GPNYTPGKKE  DLYLKPIQRT  ILMMGRYVEP  IEDVPCGNIV  GLVGVDQFLV  480
481   KTGTITTFEH  AHNMRVMKFS  VSPVVRVAVE  AKNPADLPKL  VEGLKRLAKS  DPMVQCIIEE  540
541   SGEHIIAGAG  ELHLEICLKD  LEEDHACIPI  KKSDPVVSYR  ETVSEESNVL  CLSKSPNKHN  600
601   RLYMKARPFP  DGLAEDIDKG  EVSARQELKQ  RARYLAEKYE  WDVAEARKIW  CFGPDGTGPN  660
661   ILTDITKGVQ  YLNEIKDSVV  AGFQWATKEG  ALCEENMRGV  RFDVHDVTLH  ADAIHRGGGQ  720
721   IIPTARRCLY  ASVLTAQPRL  MEPIYLVEIQ  CPEQVVGGIY  GVLNRKRGHV  FEESQVAGTP  780
781   MFVVKAYLPV  NESFGFTADL  RSNTGGQAFP  QCVFDHWQIL  PGDPFDNSSR  PSQVVAETRK  840
841   RKGLKEGIPA  LDNFLDKL  858
Nucleotide CDS Sequence (FASTA)
1     ATGGTGAACT  TCACGGTAGA  CCAGATCCGC  GCCATCATGG  ACAAGAAGGC  CAACATCCGC  60
61    AACATGTCTG  TCATCGCCCA  CGTGGACCAT  GGCAAGTCCA  CGCTGACAGA  CTCCCTGGTG  120
121   TGCAAGGCGG  GCATCATCGC  CTCGGCCCGG  GCCGGGGAGA  CACGCTTCAC  TGATACCCGG  180
181   AAGGACGAGC  AGGAGCGTTG  CATCACCATC  AAGTCAACTG  CCATCTCCCT  CTTCTACGAG  240
241   CTCTCGGAGA  ATGACTTGAA  CTTCATCAAG  CAGAGCAAGG  ACGGTGCCGG  CTTCCTCATC  300
301   AACCTCATTG  ACTCCCCCGG  GCATGTCGAC  TTCTCCTCGG  AGGTGACTGC  TGCCCTCCGA  360
361   GTCACCGATG  GCGCATTGGT  GGTGGTGGAC  TGCGTGTCAG  GCGTGTGCGT  GCAGACGGAG  420
421   ACAGTGCTGC  GGCAGGCCAT  TGCCGAGCGC  ATCAAGCCTG  TGCTGATGAT  GAACAAGATG  480
481   GACCGCGCCC  TGCTGGAGCT  GCAGCTGGAG  CCCGAGGAGC  TCTACCAGAC  TTTCCAGCGC  540
541   ATCGTGGAGA  ACGTGAACGT  CATCATCTCC  ACCTACGGCG  AGGGCGAGAG  CGGCCCCATG  600
601   GGCAACATCA  TGATCGATCC  TGTCCTCGGT  ACCGTGGGCT  TTGGGTCTGG  CCTCCACGGG  660
661   TGGGCCTTCA  CCCTGAAGCA  GTTTGCCGAG  ATGTATGTGG  CCAAGTTCGC  CGCCAAGGGG  720
721   GAGGGCCAGT  TGGGGCCTGC  CGAGCGGGCC  AAGAAAGTAG  AGGACATGAT  GAAGAAGCTG  780
781   TGGGGTGACA  GGTACTTTGA  CCCAGCCAAC  GGCAAGTTCA  GCAAGTCAGC  CACCAGCCCC  840
841   GAAGGGAAGA  AGCTGCCACG  CACCTTCTGC  CAGCTGATCC  TGGACCCCAT  CTTCAAGGTG  900
901   TTTGATGCGA  TCATGAATTT  CAAGAAAGAG  GAGACAGCAA  AACTGATAGA  GAAACTGGAC  960
961   ATCAAACTGG  ACAGCGAGGA  CAAGGACAAA  GAAGGCAAAC  CCCTGCTGAA  GGCTGTGATG  1020
1021  CGCCGCTGGC  TGCCTGCCGG  AGACGCCTTG  TTGCAGATGA  TCACCATCCA  CCTGCCCTCC  1080
1081  CCTGTGACGG  CCCAGAAGTA  CCGCTGCGAG  CTCCTGTACG  AGGGGCCCCC  GGACGACGAG  1140
1141  GCTGCCATGG  GCATTAAAAG  CTGTGACCCC  AAAGGCCCTC  TTATGATGTA  TATTTCCAAA  1200
1201  ATGGTGCCAA  CCTCCGACAA  AGGTCGGTTC  TACGCCTTTG  GACGAGTCTT  CTCGGGGCTG  1260
1261  GTCTCCACTG  GCCTGAAGGT  CAGGATCATG  GGGCCCAACT  ATACCCCTGG  GAAGAAGGAG  1320
1321  GACCTCTACC  TGAAGCCAAT  CCAGAGAACA  ATCTTGATGA  TGGGCCGCTA  CGTGGAGCCC  1380
1381  ATCGAGGATG  TGCCTTGTGG  GAACATTGTG  GGCCTCGTGG  GCGTGGACCA  GTTCCTGGTG  1440
1441  AAGACGGGCA  CCATCACCAC  CTTCGAGCAC  GCGCACAACA  TGCGGGTGAT  GAAGTTCAGC  1500
1501  GTCAGCCCTG  TTGTCAGAGT  GGCCGTGGAG  GCCAAGAACC  CGGCTGACCT  GCCCAAGCTG  1560
1561  GTGGAGGGGC  TGAAGCGGCT  GGCCAAGTCC  GACCCCATGG  TGCAGTGCAT  CATCGAGGAG  1620
1621  TCGGGAGAGC  ATATCATCGC  GGGCGCCGGC  GAGCTGCACC  TGGAGATCTG  CCTGAAGGAC  1680
1681  CTGGAGGAGG  ACCACGCCTG  CATCCCCATC  AAGAAATCTG  ACCCGGTCGT  CTCGTACCGC  1740
1741  GAGACGGTCA  GTGAAGAGTC  GAACGTGCTC  TGCCTCTCCA  AGTCCCCCAA  CAAGCACAAC  1800
1801  CGGCTGTACA  TGAAGGCGCG  GCCCTTCCCC  GACGGCCTGG  CCGAGGACAT  CGATAAAGGC  1860
1861  GAGGTGTCCG  CCCGTCAGGA  GCTCAAGCAG  CGGGCGCGCT  ACCTGGCCGA  GAAGTACGAG  1920
1921  TGGGACGTGG  CTGAGGCCCG  CAAGATCTGG  TGCTTTGGGC  CCGACGGCAC  CGGCCCCAAC  1980
1981  ATCCTCACCG  ACATCACCAA  GGGTGTGCAG  TACCTCAACG  AGATCAAGGA  CAGTGTGGTG  2040
2041  GCCGGCTTCC  AGTGGGCCAC  CAAGGAGGGC  GCACTGTGTG  AGGAGAACAT  GCGGGGTGTG  2100
2101  CGCTTCGACG  TCCACGACGT  CACCCTGCAC  GCCGACGCCA  TCCACCGCGG  AGGGGGCCAG  2160
2161  ATCATCCCCA  CAGCACGGCG  CTGCCTCTAT  GCCAGTGTGC  TGACCGCCCA  GCCACGCCTC  2220
2221  ATGGAGCCCA  TCTACCTTGT  GGAGATCCAG  TGTCCAGAGC  AGGTGGTCGG  TGGCATCTAC  2280
2281  GGGGTTTTGA  ACAGGAAGCG  GGGCCACGTG  TTCGAGGAGT  CCCAGGTGGC  CGGCACCCCC  2340
2341  ATGTTTGTGG  TCAAGGCCTA  TCTGCCCGTC  AACGAGTCCT  TTGGCTTCAC  CGCTGACCTG  2400
2401  AGGTCCAACA  CGGGCGGCCA  GGCGTTCCCC  CAGTGTGTGT  TTGACCACTG  GCAGATCCTG  2460
2461  CCCGGAGACC  CCTTCGACAA  CAGCAGCCGC  CCCAGCCAGG  TGGTGGCGGA  GACCCGCAAG  2520
2521  CGCAAGGGCC  TGAAAGAAGG  CATCCCTGCC  CTGGACAACT  TCCTGGACAA  ATTGTAG  2577

▼ KEYWORD

ID
Family
3D-structure
Acetylation
ADP-ribosylation
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Elongation factor
GTP-binding
Isopeptide bond
Methylation
Neurodegeneration
Nucleotide-binding
Nucleus
Phosphoprotein
Protein biosynthesis
Reference proteome
Spinocerebellar ataxia
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Aggresome
Cellular Component
Cytoplasm
Cellular Component
Cytosol
Cellular Component
Extracellular exosome
Cellular Component
Extracellular region
Cellular Component
Ficolin-1-rich granule lumen
Cellular Component
Membrane
Cellular Component
Membrane raft
Cellular Component
Nucleus
Cellular Component
Plasma membrane
Cellular Component
Polysomal ribosome
Cellular Component
Ribonucleoprotein complex
Cellular Component
Secretory granule lumen
Cellular Component
Synapse
Molecular Function
5S rRNA binding
Molecular Function
Actin filament binding
Molecular Function
Cadherin binding
Molecular Function
GTP binding
Molecular Function
GTPase activity
Molecular Function
P53 binding
Molecular Function
Protein kinase binding
Molecular Function
Ribosome binding
Molecular Function
RNA binding
Molecular Function
Translation elongation factor activity
Biological Process
Aging
Biological Process
Cellular response to brain-derived neurotrophic factor stimulus
Biological Process
Glial cell proliferation
Biological Process
Hematopoietic progenitor cell differentiation
Biological Process
Neutrophil degranulation
Biological Process
Positive regulation of cytoplasmic translation
Biological Process
Positive regulation of translation
Biological Process
Response to endoplasmic reticulum stress
Biological Process
Response to estradiol
Biological Process
Response to ethanol
Biological Process
Response to folic acid
Biological Process
Response to hydrogen peroxide
Biological Process
Response to ischemia
Biological Process
Skeletal muscle cell differentiation
Biological Process
Skeletal muscle contraction
Biological Process
Translational elongation

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Pat-0575Pan troglodytes100.00.01725
LLPS-Gog-0536Gorilla gorilla100.00.01725
LLPS-Pap-0855Pan paniscus99.880.01724
LLPS-Rhb-0017Rhinopithecus bieti99.880.01721
LLPS-Maf-0538Macaca fascicularis99.880.01723
LLPS-Chs-0329Chlorocebus sabaeus99.880.01723
LLPS-Cea-0115Cercocebus atys99.880.01723
LLPS-Mal-0614Mandrillus leucophaeus99.880.01723
LLPS-Paa-0240Papio anubis99.880.01723
LLPS-Man-2136Macaca nemestrina99.770.01717
LLPS-Mam-3067Macaca mulatta99.770.01722
LLPS-Ict-0159Ictidomys tridecemlineatus99.650.01719
LLPS-Sus-0606Sus scrofa99.650.01719
LLPS-Dio-1342Dipodomys ordii99.650.01719
LLPS-Urm-0602Ursus maritimus99.530.01695
LLPS-Loa-3084Loxodonta africana99.530.01718
LLPS-Caf-1491Canis familiaris99.530.01721
LLPS-Aon-0864Aotus nancymaae99.530.01716
LLPS-Caj-2447Callithrix jacchus99.530.01718
LLPS-Fec-0242Felis catus99.420.01717
LLPS-Mea-0508Mesocricetus auratus99.30.01714
LLPS-Bot-0460Bos taurus99.30.01716
LLPS-Ova-0956Ovis aries99.30.01714
LLPS-Aim-0836Ailuropoda melanoleuca99.30.01712
LLPS-Eqc-0311Equus caballus99.180.01716
LLPS-Fud-2072Fukomys damarensis99.180.01712
LLPS-Ran-1104Rattus norvegicus99.180.01713
LLPS-Mum-0740Mus musculus99.070.01711
LLPS-Sah-1847Sarcophilus harrisii98.950.01710
LLPS-Myl-0922Myotis lucifugus98.840.01702
LLPS-Otg-2295Otolemur garnettii98.830.01706
LLPS-Mod-2574Monodelphis domestica98.60.01706
LLPS-Cas-0268Carlito syrichta98.370.01702
LLPS-Tag-0557Taeniopygia guttata98.240.01100
LLPS-Mup-1649Mustela putorius furo98.040.01495
LLPS-Pes-1569Pelodiscus sinensis98.020.01696
LLPS-Fia-0169Ficedula albicollis97.780.01691
LLPS-Gaga-0921Gallus gallus97.550.01690
LLPS-Meg-0906Meleagris gallopavo96.760.01680
LLPS-Xet-1108Xenopus tropicalis95.920.01667
LLPS-Anc-0428Anolis carolinensis94.530.01646
LLPS-Cap-1194Cavia porcellus94.170.01603
LLPS-Leo-0291Lepisosteus oculatus94.060.01644
LLPS-Scf-2804Scleropages formosus93.940.01634
LLPS-Asm-0568Astyanax mexicanus93.00.01614
LLPS-Dar-1047Danio rerio92.450.01615
LLPS-Orn-1607Oreochromis niloticus92.310.01608
LLPS-Nol-1342Nomascus leucogenys92.310.01229
LLPS-Pof-1705Poecilia formosa92.070.01607
LLPS-Lac-0881Latimeria chalumnae92.070.01607
LLPS-Orl-0912Oryzias latipes91.840.01600
LLPS-Icp-0616Ictalurus punctatus91.520.01605
LLPS-Scm-0622Scophthalmus maximus90.440.01583
LLPS-Gaa-3306Gasterosteus aculeatus89.730.01560
LLPS-Xim-2552Xiphophorus maculatus88.910.01588
LLPS-Tar-1517Takifugu rubripes83.310.01509
LLPS-Cae-0399Caenorhabditis elegans78.740.01379
LLPS-Drm-0997Drosophila melanogaster78.310.01353
LLPS-Cis-0020Ciona savignyi73.50.01260
LLPS-Tum-0486Tuber melanosporum73.120.0 725
LLPS-Cii-0975Ciona intestinalis72.990.01232
LLPS-Nef-0123Neosartorya fischeri68.160.01144
LLPS-Spr-1244Sporisorium reilianum67.830.01165
LLPS-Fuv-0583Fusarium verticillioides67.830.01183
LLPS-Asc-0875Aspergillus clavatus67.810.01136
LLPS-Pug-0551Puccinia graminis67.60.01178
LLPS-Fus-0034Fusarium solani67.60.01159
LLPS-Usm-0628Ustilago maydis67.60.01159
LLPS-Asfu-0526Aspergillus fumigatus67.560.01157
LLPS-Phn-0292Phaeosphaeria nodorum67.560.01172
LLPS-Gag-0333Gaeumannomyces graminis67.260.01143
LLPS-Map-0627Magnaporthe poae67.260.01138
LLPS-Trr-0077Trichoderma reesei67.250.01162
LLPS-Asni-0103Aspergillus niger67.090.01156
LLPS-Trv-0137Trichoderma virens67.020.01157
LLPS-Nec-0540Neurospora crassa67.020.01157
LLPS-Pytr-0491Pyrenophora triticirepentis66.980.01138
LLPS-Asn-0118Aspergillus nidulans66.980.01164
LLPS-Pyt-0473Pyrenophora teres66.980.01156
LLPS-Cogr-0234Colletotrichum graminicola66.790.01113
LLPS-Beb-0543Beauveria bassiana66.670.01152
LLPS-Coo-0941Colletotrichum orbiculare66.670.01129
LLPS-Mao-0183Magnaporthe oryzae66.670.01160
LLPS-Asg-0432Ashbya gossypii66.670.01154
LLPS-Zyt-0355Zymoseptoria tritici66.630.01156
LLPS-Crn-1121Cryptococcus neoformans66.550.01130
LLPS-Sac-0623Saccharomyces cerevisiae66.20.01147
LLPS-Cog-0843Colletotrichum gloeosporioides66.160.01126
LLPS-Miv-0742Microbotryum violaceum66.080.01146
LLPS-Mel-0457Melampsora laricipopulina66.080.01146
LLPS-Ved-0105Verticillium dahliae65.970.01144
LLPS-Yal-0083Yarrowia lipolytica65.970.01141
LLPS-Aso-1066Aspergillus oryzae65.850.01118
LLPS-Dos-0513Dothistroma septosporum65.820.01148
LLPS-Asf-0720Aspergillus flavus65.820.01130
LLPS-Kop-0025Komagataella pastoris65.620.01137
LLPS-Scj-0377Schizosaccharomyces japonicus65.620.01143
LLPS-Put-0363Puccinia triticina65.350.01092
LLPS-Blg-0465Blumeria graminis65.190.01134
LLPS-Abg-0089Absidia glauca65.030.01117
LLPS-Gas-0717Galdieria sulphuraria64.770.01088
LLPS-Scp-0657Schizosaccharomyces pombe64.570.01127
LLPS-Scc-1243Schizosaccharomyces cryophilus63.990.01117
LLPS-Gor-0837Gossypium raimondii63.650.01080
LLPS-Mae-0298Manihot esculenta63.570.01078
LLPS-Thc-0787Theobroma cacao63.570.01075
LLPS-Via-0989Vigna angularis63.570.01051
LLPS-Phv-1918Phaseolus vulgaris63.530.01079
LLPS-Amt-0014Amborella trichopoda63.340.01073
LLPS-Viv-0212Vitis vinifera63.30.01073
LLPS-Cus-0423Cucumis sativus63.30.01082
LLPS-Vir-0971Vigna radiata63.30.01073
LLPS-Pot-0432Populus trichocarpa63.230.01071
LLPS-Glm-1988Glycine max63.230.01075
LLPS-Sem-0615Selaginella moellendorffii63.230.01072
LLPS-Met-0676Medicago truncatula63.180.01077
LLPS-Ors-1672Oryza sativa63.070.01074
LLPS-Orp-0351Oryza punctata63.070.01074
LLPS-Orm-0815Oryza meridionalis63.070.01072
LLPS-Prp-1425Prunus persica63.070.01075
LLPS-Org-1657Oryza glaberrima63.070.01074
LLPS-Orb-1046Oryza barthii63.070.01074
LLPS-Ori-0963Oryza indica63.070.01074
LLPS-Orni-0495Oryza nivara63.070.01074
LLPS-Orgl-0807Oryza glumaepatula63.070.01074
LLPS-Orbr-1598Oryza brachyantha62.990.01067
LLPS-Orr-1895Oryza rufipogon62.950.01072
LLPS-Sol-2037Solanum lycopersicum62.950.01073
LLPS-Hea-0186Helianthus annuus62.830.01065
LLPS-Mua-0678Musa acuminata62.830.01071
LLPS-Sob-1953Sorghum bicolor62.760.01066
LLPS-Dac-0570Daucus carota62.720.01075
LLPS-Chc-0004Chondrus crispus62.690.01065
LLPS-Brd-0362Brachypodium distachyon62.650.01063
LLPS-Php-0356Physcomitrella patens62.60.01067
LLPS-Osl-0814Ostreococcus lucimarinus62.590.01061
LLPS-Zem-1438Zea mays62.530.01063
LLPS-Sei-1936Setaria italica62.490.01066
LLPS-Hov-0232Hordeum vulgare62.410.01066
LLPS-Nia-0144Nicotiana attenuata62.370.01065
LLPS-Tru-0533Triticum urartu62.370.01058
LLPS-Tra-1101Triticum aestivum62.30.01060
LLPS-Brr-0993Brassica rapa62.250.01069
LLPS-Arl-1492Arabidopsis lyrata62.180.01065
LLPS-Chr-1271Chlamydomonas reinhardtii62.150.01053
LLPS-Lep-0472Leersia perrieri62.140.01062
LLPS-Art-0251Arabidopsis thaliana62.060.01059
LLPS-Bro-1377Brassica oleracea61.790.01063
LLPS-Scs-0368Sclerotinia sclerotiorum61.320.01038
LLPS-Brn-1394Brassica napus59.120.0 990
LLPS-Ast-1094Aspergillus terreus58.420.0 964
LLPS-Sot-0276Solanum tuberosum41.20.0 640
LLPS-Coc-0693Corchorus capsularis39.930.0 628
LLPS-Orc-0110Oryctolagus cuniculus39.060.0 624