• Disorder▼
  • Domain▼
  • PTM▼
  • Variation▼
  • Mutation▼
  • Interaction▼
  • Disease▼
  • Drug▼
  • Physicochemical▼
  • Function▼
  • Proteomics▼
  • Structure▼
  • Localization▼
  • Expression▼
  • Element▼
  • Methylation▼

LLPS-Caj-0635
RNF168

Integrated Annotations

▼ OVERVIEW

Status: Unreviewed
Protein Name: E3 ubiquitin-protein ligase RNF168; RING finger protein 168; RING-type E3 ubiquitin transferase RNF168
Gene Name: RNF168
Ensembl Gene: ENSCJAG00000001693.3
Ensembl Protein: ENSCJAP00000003034.3
Organism: Callithrix jacchus
Taxa ID: 9483
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Chromatin, PML nuclear bodyPredicted from orthologs(View)

▼ FUNCTION

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).

▼ CROSS REFERENCE

DatabaseNucleotide IDProtein ID
EnsemblENSCJAT00000003203.3ENSCJAP00000003034.3
UniProtF7GIE2, F7GIE2_CALJA

▼ SEQUENCE

Protein Sequence (FASTA)
1     MAVPKAAVPS  LSECQCGICM  EILFEPVTLP  CNHTLCKPCF  QSTVEKASLC  CPFCRRRVSS  60
61    WTRYHTRRNS  LVNLELWTII  QKHYARECKL  RAAGQESEEV  AYDYQPVRLL  SKPGELRREY  120
121   EEEISKVEAE  RRATEEEENK  ASEEYIQRLL  AEEEEEDRRQ  AEKRRREMEE  QLKNDEELAR  180
181   KLSVDINNFC  EGSILVSPLN  SRKSDPVRAK  SEKKSKNKQR  NIGDIQKYLT  PKSQFGSASH  240
241   SEVAQEVREN  SISKDIDSSD  IKSSTWQDTE  IEDMPTLSPQ  ICLGIGEKGA  DSSVESPMPW  300
301   LCACGAELYH  ERKVKAGPNN  HDTTCVLTPE  GPETRDCDSK  ETAVVPIGRT  ESGCTTSGIT  360
361   QANRNNTGET  ENEESCLLIS  KDISKRKNQE  SSFEAVKDAC  FSAKRRKMFP  SSSSDQEETE  420
421   TNFTQKLIDL  EHLLLERHKQ  EEQDRLLALQ  LQKEVDKEQT  KPNRQKGSPD  EYHLRATSLP  480
481   PDKVRNGQRK  NSKDRNFKRQ  TDTEHPKPER  GSRDENRQLS  VRMQLRHSVN  GRKTPNSTRD  540
541   PCNVSRSAHS  LQPNISQKSI  YQMFHRCTK  569
Nucleotide CDS Sequence (FASTA)
1     ATGGCTGTAC  CCAAAGCCGC  CGTCCCTTCG  CTCTCTGAGT  GCCAGTGCGG  GATCTGCATG  60
61    GAAATCCTTT  TCGAGCCCGT  CACCCTCCCG  TGTAACCACA  CGCTCTGTAA  ACCGTGCTTC  120
121   CAGTCGACTG  TCGAAAAGGC  AAGTTTATGC  TGTCCCTTCT  GTCGTCGCCG  CGTATCTTCG  180
181   TGGACTCGTT  ACCACACCCG  AAGAAATTCC  CTTGTCAACC  TGGAACTGTG  GACGATAATT  240
241   CAAAAACACT  ATGCAAGGGA  GTGCAAGCTT  AGAGCCGCTG  GGCAAGAATC  GGAGGAAGTC  300
301   GCTTATGACT  ATCAGCCAGT  TCGTCTGTTA  AGTAAACCTG  GGGAATTGAG  AAGAGAATAC  360
361   GAAGAGGAAA  TAAGCAAGGT  GGAGGCAGAG  CGACGAGCCA  CTGAGGAAGA  AGAAAACAAA  420
421   GCCAGTGAAG  AATACATACA  GAGGTTATTG  GCAGAGGAGG  AAGAGGAGGA  CAGAAGACAG  480
481   GCAGAAAAAA  GGCGAAGAGA  GATGGAAGAA  CAACTGAAAA  ATGATGAAGA  ACTGGCAAGA  540
541   AAGTTAAGCG  TTGATATTAA  TAATTTCTGT  GAGGGAAGTA  TCTTGGTTTC  TCCCTTGAAT  600
601   TCCAGAAAAT  CTGATCCAGT  TAGAGCCAAG  TCTGAAAAGA  AAAGTAAGAA  CAAACAAAGA  660
661   AACATTGGAG  ATATTCAGAA  GTATTTGACA  CCTAAATCTC  AGTTTGGGTC  AGCCTCACAC  720
721   TCTGAAGTTG  CACAAGAAGT  CAGAGAAAAC  TCCATATCTA  AGGACATCGA  CAGTAGTGAT  780
781   ATCAAAAGCT  CAACATGGCA  AGACACAGAA  ATAGAAGATA  TGCCAACACT  TTCTCCACAG  840
841   ATATGCCTTG  GAATTGGAGA  AAAAGGTGCA  GATTCTTCAG  TGGAGTCCCC  CATGCCATGG  900
901   TTATGTGCCT  GTGGTGCTGA  ATTGTACCAC  GAAAGAAAAG  TCAAAGCAGG  ACCAAACAAT  960
961   CATGATACAA  CGTGTGTCTT  AACTCCCGAA  GGACCTGAAA  CCAGAGATTG  TGACTCTAAA  1020
1021  GAAACTGCAG  TTGTGCCTAT  TGGCAGAACA  GAGAGTGGGT  GCACCACATC  AGGGATTACA  1080
1081  CAGGCAAACA  GAAACAACAC  AGGTGAGACA  GAAAATGAGG  AGTCTTGCCT  ACTGATCAGT  1140
1141  AAGGATATTT  CCAAAAGAAA  AAACCAAGAA  TCTTCCTTTG  AAGCAGTCAA  GGATGCATGC  1200
1201  TTTTCTGCAA  AAAGAAGGAA  GATGTTCCCC  AGCTCTTCCT  CAGATCAAGA  GGAAACAGAA  1260
1261  ACAAACTTTA  CCCAAAAACT  GATAGATTTG  GAGCATCTAC  TGTTAGAGAG  ACATAAACAA  1320
1321  GAAGAACAGG  ACAGGTTATT  GGCATTACAA  CTTCAGAAGG  AAGTGGACAA  AGAACAAACG  1380
1381  AAGCCAAACC  GGCAAAAAGG  ATCCCCAGAT  GAGTATCACT  TACGTGCTAC  ATCCTTACCT  1440
1441  CCAGACAAAG  TGCGAAATGG  ACAGAGGAAG  AATTCCAAAG  ATAGGAACTT  CAAAAGGCAA  1500
1501  ACTGATACAG  AACACCCAAA  ACCTGAGAGA  GGCTCAAGGG  ATGAAAATAG  GCAGCTGTCT  1560
1561  GTTAGGATGC  AGCTGAGGCA  TTCAGTTAAT  GGAAGAAAGA  CGCCAAATTC  TACTAGAGAT  1620
1621  CCTTGTAATG  TATCCAGAAG  TGCTCATTCC  CTGCAGCCAA  ACATTTCACA  GAAAAGCATT  1680
1681  TATCAGATGT  TTCACAGATG  TACAAAGTAA  1710

▼ KEYWORD

ID
Family
Chromatin regulator
Coiled coil
Complete proteome
DNA damage
DNA repair
Metal-binding
Nucleus
Reference proteome
Transferase
Ubl conjugation
Ubl conjugation pathway
Zinc
Zinc-finger

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytosol
Cellular Component
Nucleoplasm
Cellular Component
Site of double-strand break
Cellular Component
Ubiquitin ligase complex
Molecular Function
Histone binding
Molecular Function
K63-linked polyubiquitin modification-dependent protein binding
Molecular Function
Metal ion binding
Molecular Function
Nucleosome binding
Molecular Function
Ubiquitin binding
Molecular Function
Ubiquitin-protein transferase activity
Biological Process
Double-strand break repair
Biological Process
Histone H2A K63-linked ubiquitination
Biological Process
Histone H2A monoubiquitination
Biological Process
Histone H2A-K13 ubiquitination
Biological Process
Histone H2A-K15 ubiquitination
Biological Process
Negative regulation of transcription elongation from RNA polymerase II promoter
Biological Process
Positive regulation of DNA repair
Biological Process
Response to ionizing radiation
Biological Process
Ubiquitin-dependent protein catabolic process

▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Aon-2352Aotus nancymaae93.150.0 943
LLPS-Nol-3731Nomascus leucogenys85.110.0 833
LLPS-Hos-2753Homo sapiens84.590.0 836
LLPS-Pap-0507Pan paniscus84.410.0 828
LLPS-Poa-2748Pongo abelii84.060.0 827
LLPS-Gog-0718Gorilla gorilla83.580.0 635
LLPS-Cas-0360Carlito syrichta83.077e-76 247
LLPS-Ict-3173Ictidomys tridecemlineatus82.545e-75 243
LLPS-Rhb-4384Rhinopithecus bieti81.960.0 798
LLPS-Chs-2638Chlorocebus sabaeus80.560.0 791
LLPS-Mam-0417Macaca mulatta80.390.0 792
LLPS-Maf-1674Macaca fascicularis80.040.0 791
LLPS-Man-3855Macaca nemestrina80.040.0 790
LLPS-Cea-1746Cercocebus atys79.860.0 792
LLPS-Paa-4454Papio anubis79.510.0 784
LLPS-Mal-1525Mandrillus leucophaeus78.460.0 759
LLPS-Tut-0750Tursiops truncatus78.280.0 727
LLPS-Eqc-1912Equus caballus77.660.0 731
LLPS-Urm-2039Ursus maritimus77.060.0 726
LLPS-Fec-3797Felis catus75.780.0 718
LLPS-Caf-2468Canis familiaris75.310.0 691
LLPS-Otg-2512Otolemur garnettii75.220.0 714
LLPS-Aim-0945Ailuropoda melanoleuca74.960.0 701
LLPS-Mup-0957Mustela putorius furo74.520.0 696
LLPS-Myl-2257Myotis lucifugus74.260.0 692
LLPS-Sus-1694Sus scrofa73.030.0 708
LLPS-Bot-1448Bos taurus72.080.0 671
LLPS-Ova-0440Ovis aries71.550.0 656
LLPS-Loa-2471Loxodonta africana69.270.0 678
LLPS-Orc-1964Oryctolagus cuniculus68.480.0 648
LLPS-Pat-3076Pan troglodytes68.420.0 639
LLPS-Anc-2295Anolis carolinensis66.946e-48 181
LLPS-Ora-2935Ornithorhynchus anatinus66.146e-49 171
LLPS-Fud-3515Fukomys damarensis65.380.0 568
LLPS-Cap-0381Cavia porcellus62.917e-177 520
LLPS-Mea-1870Mesocricetus auratus62.210.0 546
LLPS-Ran-1543Rattus norvegicus61.430.0 570
LLPS-Mum-1890Mus musculus61.40.0 558
LLPS-Tag-1257Taeniopygia guttata57.072e-46 167
LLPS-Sah-1526Sarcophilus harrisii52.574e-140 425
LLPS-Mod-2187Monodelphis domestica51.124e-125 387
LLPS-Gaa-1914Gasterosteus aculeatus50.854e-34 134
LLPS-Ten-2956Tetraodon nigroviridis50.03e-32 131
LLPS-Tar-1934Takifugu rubripes49.171e-32 134
LLPS-Pof-3319Poecilia formosa48.313e-31 130
LLPS-Orn-0371Oreochromis niloticus44.932e-33 136
LLPS-Fia-0761Ficedula albicollis43.271e-91 299
LLPS-Asm-0193Astyanax mexicanus43.135e-35 141
LLPS-Anp-1543Anas platyrhynchos42.54e-79 265
LLPS-Pes-0732Pelodiscus sinensis42.232e-91 300
LLPS-Icp-3653Ictalurus punctatus41.741e-35 143
LLPS-Dar-2417Danio rerio41.363e-37 148
LLPS-Phv-1225Phaseolus vulgaris40.969e-1168.2
LLPS-Prp-1636Prunus persica40.628e-1065.9
LLPS-Scf-2304Scleropages formosus40.512e-35 142
LLPS-Thc-0644Theobroma cacao40.244e-1168.9
LLPS-Brr-3063Brassica rapa40.02e-0860.5
LLPS-Bro-2420Brassica oleracea40.03e-0860.1
LLPS-Meg-2463Meleagris gallopavo39.742e-85 283
LLPS-Gaga-2163Gallus gallus39.447e-82 275
LLPS-Xet-1702Xenopus tropicalis39.43e-67 233
LLPS-Dac-1130Daucus carota39.244e-1065.5
LLPS-Arl-2484Arabidopsis lyrata39.023e-0860.8
LLPS-Mae-2147Manihot esculenta39.024e-1066.2
LLPS-Viv-2498Vitis vinifera39.021e-0964.3
LLPS-Sot-0543Solanum tuberosum38.743e-1375.5
LLPS-Vir-1992Vigna radiata38.543e-1168.9
LLPS-Nia-2384Nicotiana attenuata38.141e-1170.5
LLPS-Sol-0816Solanum lycopersicum38.05e-1375.5
LLPS-Gor-1240Gossypium raimondii37.115e-1168.9
LLPS-Cii-0130Ciona intestinalis37.043e-1787.0
LLPS-Via-2392Vigna angularis36.469e-1168.2
LLPS-Leo-0509Lepisosteus oculatus36.275e-50 185
LLPS-Orp-1407Oryza punctata36.267e-0962.0
LLPS-Hea-0678Helianthus annuus35.656e-1168.6
LLPS-Lac-2468Latimeria chalumnae35.486e-56 201
LLPS-Met-2400Medicago truncatula35.199e-1168.2
LLPS-Orgl-0315Oryza glumaepatula35.162e-0860.8
LLPS-Cis-1342Ciona savignyi34.826e-1682.0
LLPS-Glm-2457Glycine max34.786e-1168.6
LLPS-Orr-2045Oryza rufipogon34.414e-0653.5
LLPS-Cus-1396Cucumis sativus34.42e-1170.1
LLPS-Php-0079Physcomitrella patens34.181e-0861.6
LLPS-Zem-0411Zea mays34.05e-0962.4
LLPS-Amt-1173Amborella trichopoda32.943e-0757.4
LLPS-Brn-0712Brassica napus32.932e-0758.2
LLPS-Pot-2179Populus trichocarpa32.03e-0654.3
LLPS-Art-2679Arabidopsis thaliana31.716e-0756.6
LLPS-Tra-2973Triticum aestivum30.931e-0655.5
LLPS-Coc-0716Corchorus capsularis30.593e-0654.3
LLPS-Hov-2053Hordeum vulgare30.096e-0756.6
LLPS-Brd-2249Brachypodium distachyon29.91e-0655.1
LLPS-Mua-0449Musa acuminata29.296e-0756.6
LLPS-Tru-0682Triticum urartu28.875e-0653.5
LLPS-Orni-1968Oryza nivara28.284e-0653.9
LLPS-Ori-0471Oryza indica28.284e-0653.9
LLPS-Orb-1495Oryza barthii28.284e-0653.9
LLPS-Sei-1904Setaria italica27.848e-0653.1
LLPS-Sob-1959Sorghum bicolor27.15e-0653.5