LLPS-Bot-3431
PAF1
Integrated Annotations
▼ OVERVIEW
| Status: | Unreviewed |
| Protein Name: | RNA polymerase II-associated factor 1 homolog |
| Gene Name: | PAF1 |
| Ensembl Gene: | ENSBTAG00000020428.6 |
| Ensembl Protein: | ENSBTAP00000027224.6 |
| Organism: | Bos taurus |
| Taxa ID: | 9913 |
| LLPS Type: | LLPS regulator |
▼ Classification
Condensates:
| Condensate | Evidence | Orthologs |
|---|---|---|
| Nucleolus, Centrosome/Spindle pole body, P-body | Predicted from orthologs | (View) |
▼ FUNCTION
| Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors (By similarity). |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENSBTAT00000027224.6 | ENSBTAP00000027224.6 |
| UniProt | Q2KJ14, PAF1_BOVIN | |
| GeneBank | BC105570 | AAI05571.1 |
| RefSeq | NM_001046293.1 | NP_001039758.1 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MAPTIQTQAQ REDGHRPNSH RTLPERSGVV CRVKYCNSLP DIPFDPKFIT YPFDQNRFVQ 60 61 YKATSLEKQH KHDLLTEPDL GVTIDLINPD TYRIDPNVLL DPADEKLLEE EIQAPTSSKR 120 121 SQQHAKVVPW MRKTEYISTE FNRYGISNEK PEVKIGVSVK QQFTEEEIYK DRDSQITAIE 180 181 KTFEDAQKSI SQHYSKPRVT PVEVMPVFPD FKMWINPCAQ VIFDSDPAPK DTSGAAALEM 240 241 MSQAMIRGMM DEEGNQFVAY FLPVEETLKK RKRDQEEEMD YAPDDVYDYK IAREYNWNVK 300 301 NKASKGYEEN YFFIFREGDG VYYNELETRV RLSKRRAKAG VQSGTNALLV VKHRDMNEKE 360 361 LEAQEARKAQ LENHEPEEEE EEEMETEEKE AGASDEEREK GSSSEKEGSE DERSGSESER 420 421 EEGDRDEASD KSGSGEDESS EDEARAARDK EEIFGSDADS EDDADSDDED RGRARGSDND 480 481 SDSGSDGGGQ RSRSHSRSRS ASPFPSGSEH SAQEDGSEAA ASDSSEADSD SD 532 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGGCGCCCA CTATCCAGAC CCAGGCCCAG CGCGAGGATG GCCACAGGCC CAATTCTCAC 60 61 CGGACTTTGC CTGAGAGGTC TGGAGTGGTC TGCCGAGTCA AGTACTGCAA CAGCCTCCCT 120 121 GACATCCCCT TCGACCCGAA GTTCATCACC TATCCCTTCG ACCAGAACAG GTTTGTTCAG 180 181 TACAAAGCAA CTTCCCTGGA AAAACAGCAC AAACATGATC TCTTGACTGA GCCAGACCTG 240 241 GGAGTTACCA TTGACCTCAT CAACCCTGAT ACCTACCGCA TCGACCCCAA TGTACTCCTA 300 301 GACCCAGCTG ATGAGAAGCT TCTGGAAGAG GAGATTCAGG CCCCCACCAG TTCCAAGAGA 360 361 TCCCAGCAGC ACGCAAAGGT GGTGCCATGG ATGCGGAAGA CAGAGTACAT CTCCACGGAA 420 421 TTCAACCGTT ATGGCATTTC CAATGAGAAG CCTGAGGTCA AGATTGGGGT TTCTGTGAAG 480 481 CAGCAATTCA CTGAGGAGGA AATATACAAA GACAGGGATA GCCAGATCAC AGCTATTGAG 540 541 AAGACTTTTG AGGATGCCCA GAAATCTATC TCCCAGCATT ATAGCAAGCC CCGAGTGACA 600 601 CCAGTGGAGG TCATGCCTGT CTTTCCAGAT TTTAAGATGT GGATCAACCC TTGCGCTCAG 660 661 GTAATCTTTG ACTCAGACCC CGCCCCCAAG GACACAAGTG GTGCAGCTGC ATTGGAGATG 720 721 ATGTCTCAGG CCATGATCAG GGGCATGATG GATGAGGAAG GGAACCAGTT TGTGGCTTAC 780 781 TTTCTGCCTG TGGAGGAAAC ACTGAAGAAA CGAAAACGGG ACCAGGAAGA GGAGATGGAT 840 841 TATGCACCAG ATGATGTGTA TGACTACAAG ATTGCTCGAG AGTACAACTG GAATGTGAAG 900 901 AACAAGGCTA GCAAGGGCTA TGAGGAAAAC TACTTCTTTA TCTTCCGAGA GGGTGATGGT 960 961 GTTTACTACA ATGAATTGGA GACCAGGGTT CGCCTGAGTA AGCGCCGAGC CAAGGCCGGG 1020 1021 GTTCAGTCGG GTACCAATGC CCTGCTTGTG GTCAAACACC GGGACATGAA TGAGAAGGAA 1080 1081 TTAGAAGCCC AGGAGGCACG GAAGGCTCAG CTGGAGAACC ATGAACCCGA GGAGGAAGAA 1140 1141 GAGGAGGAAA TGGAGACAGA AGAGAAAGAA GCTGGGGCCT CAGATGAGGA ACGAGAGAAG 1200 1201 GGCAGCAGCA GTGAGAAGGA AGGCAGCGAG GATGAGCGCT CTGGCAGTGA GAGTGAACGG 1260 1261 GAGGAGGGTG ACAGGGATGA GGCGAGTGAC AAGAGTGGCA GCGGCGAGGA TGAAAGCAGT 1320 1321 GAGGATGAGG CCCGGGCCGC CAGAGACAAA GAGGAGATCT TCGGCAGTGA TGCTGATTCG 1380 1381 GAGGATGACG CTGACTCTGA TGATGAGGAC AGAGGACGGG CTCGTGGCAG TGATAATGAC 1440 1441 TCAGACAGTG GCAGTGATGG GGGTGGCCAG CGGAGCCGGA GCCACAGCCG GAGCCGGAGC 1500 1501 GCCAGTCCCT TCCCCAGTGG CAGCGAGCAT TCTGCTCAGG AGGATGGCAG TGAAGCTGCA 1560 1561 GCTTCTGATT CCAGTGAAGC TGACAGTGAC AGTGACTGA 1599 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cdc73/Paf1 complex | |
Cellular Component | Cell junction | |
Cellular Component | Cytoplasm | |
Cellular Component | Membrane | |
Cellular Component | Transcriptionally active chromatin | |
Molecular Function | Chromatin binding | |
Molecular Function | RNA polymerase II complex binding | |
Biological Process | Cellular response to lipopolysaccharide | |
Biological Process | Endodermal cell fate commitment | |
Biological Process | Histone H2B ubiquitination | |
Biological Process | Histone monoubiquitination | |
Biological Process | MRNA polyadenylation | |
Biological Process | Negative regulation of myeloid cell differentiation | |
Biological Process | Negative regulation of transcription by RNA polymerase II | |
Biological Process | Nucleosome positioning | |
Biological Process | Positive regulation of cell cycle G1/S phase transition | |
Biological Process | Positive regulation of histone methylation | |
Biological Process | Positive regulation of mRNA 3'-end processing | |
Biological Process | Positive regulation of transcription by RNA polymerase II | |
Biological Process | Positive regulation of transcription elongation from RNA polymerase II promoter | |
Biological Process | Protein localization to nucleus | |
Biological Process | Stem cell population maintenance | |
Biological Process | Transcription elongation from RNA polymerase II promoter | |
Biological Process | Wnt signaling pathway |
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Ran-1298 | Rattus norvegicus | 100.0 | 0.0 | 789 |
| LLPS-Mal-1979 | Mandrillus leucophaeus | 100.0 | 0.0 | 788 |
| LLPS-Paa-3300 | Papio anubis | 100.0 | 0.0 | 788 |
| LLPS-Mup-2534 | Mustela putorius furo | 100.0 | 0.0 | 787 |
| LLPS-Aim-2527 | Ailuropoda melanoleuca | 100.0 | 0.0 | 788 |
| LLPS-Ova-2017 | Ovis aries | 100.0 | 0.0 | 788 |
| LLPS-Myl-0864 | Myotis lucifugus | 100.0 | 0.0 | 786 |
| LLPS-Tut-1188 | Tursiops truncatus | 100.0 | 0.0 | 787 |
| LLPS-Dio-0445 | Dipodomys ordii | 100.0 | 0.0 | 706 |
| LLPS-Gog-2606 | Gorilla gorilla | 100.0 | 0.0 | 788 |
| LLPS-Fud-1912 | Fukomys damarensis | 100.0 | 0.0 | 790 |
| LLPS-Caj-2289 | Callithrix jacchus | 100.0 | 0.0 | 788 |
| LLPS-Maf-2951 | Macaca fascicularis | 100.0 | 0.0 | 788 |
| LLPS-Mum-3361 | Mus musculus | 100.0 | 0.0 | 789 |
| LLPS-Chs-2883 | Chlorocebus sabaeus | 100.0 | 0.0 | 788 |
| LLPS-Sus-1993 | Sus scrofa | 100.0 | 0.0 | 788 |
| LLPS-Cea-0828 | Cercocebus atys | 100.0 | 0.0 | 788 |
| LLPS-Aon-0510 | Aotus nancymaae | 100.0 | 0.0 | 786 |
| LLPS-Nol-1225 | Nomascus leucogenys | 100.0 | 0.0 | 789 |
| LLPS-Ict-0228 | Ictidomys tridecemlineatus | 100.0 | 0.0 | 788 |
| LLPS-Poa-1242 | Pongo abelii | 100.0 | 0.0 | 788 |
| LLPS-Caf-3368 | Canis familiaris | 100.0 | 0.0 | 780 |
| LLPS-Cap-1256 | Cavia porcellus | 100.0 | 0.0 | 788 |
| LLPS-Eqc-1553 | Equus caballus | 100.0 | 0.0 | 780 |
| LLPS-Mam-0059 | Macaca mulatta | 100.0 | 0.0 | 788 |
| LLPS-Rhb-0738 | Rhinopithecus bieti | 100.0 | 0.0 | 787 |
| LLPS-Hos-1164 | Homo sapiens | 100.0 | 0.0 | 788 |
| LLPS-Pat-2698 | Pan troglodytes | 100.0 | 0.0 | 788 |
| LLPS-Urm-1811 | Ursus maritimus | 100.0 | 0.0 | 781 |
| LLPS-Fec-0823 | Felis catus | 100.0 | 0.0 | 788 |
| LLPS-Man-2347 | Macaca nemestrina | 100.0 | 0.0 | 788 |
| LLPS-Loa-1823 | Loxodonta africana | 100.0 | 0.0 | 782 |
| LLPS-Cas-1306 | Carlito syrichta | 99.73 | 0.0 | 788 |
| LLPS-Otg-1209 | Otolemur garnettii | 99.73 | 0.0 | 777 |
| LLPS-Orc-0802 | Oryctolagus cuniculus | 99.73 | 0.0 | 778 |
| LLPS-Pap-2031 | Pan paniscus | 99.73 | 0.0 | 785 |
| LLPS-Mea-0082 | Mesocricetus auratus | 97.86 | 0.0 | 757 |
| LLPS-Mod-0045 | Monodelphis domestica | 97.04 | 0.0 | 764 |
| LLPS-Pes-2612 | Pelodiscus sinensis | 96.67 | 0.0 | 676 |
| LLPS-Meg-1386 | Meleagris gallopavo | 96.2 | 0.0 | 640 |
| LLPS-Sah-2142 | Sarcophilus harrisii | 94.34 | 0.0 | 744 |
| LLPS-Gaga-3189 | Gallus gallus | 93.82 | 0.0 | 727 |
| LLPS-Anc-1325 | Anolis carolinensis | 93.48 | 1e-119 | 362 |
| LLPS-Lac-2094 | Latimeria chalumnae | 93.26 | 0.0 | 741 |
| LLPS-Orn-0743 | Oreochromis niloticus | 91.94 | 0.0 | 726 |
| LLPS-Tar-1017 | Takifugu rubripes | 91.67 | 0.0 | 721 |
| LLPS-Leo-1093 | Lepisosteus oculatus | 91.64 | 0.0 | 731 |
| LLPS-Pof-2239 | Poecilia formosa | 91.64 | 0.0 | 723 |
| LLPS-Xim-1806 | Xiphophorus maculatus | 91.13 | 0.0 | 721 |
| LLPS-Ten-0461 | Tetraodon nigroviridis | 90.86 | 0.0 | 714 |
| LLPS-Gaa-1690 | Gasterosteus aculeatus | 90.59 | 0.0 | 719 |
| LLPS-Scf-0173 | Scleropages formosus | 90.57 | 0.0 | 720 |
| LLPS-Orl-0088 | Oryzias latipes | 90.32 | 0.0 | 717 |
| LLPS-Asm-0255 | Astyanax mexicanus | 90.3 | 0.0 | 718 |
| LLPS-Scm-0506 | Scophthalmus maximus | 89.76 | 0.0 | 713 |
| LLPS-Icp-0542 | Ictalurus punctatus | 81.59 | 0.0 | 602 |
| LLPS-Dar-1451 | Danio rerio | 80.66 | 2e-150 | 444 |
| LLPS-Xet-1960 | Xenopus tropicalis | 79.73 | 0.0 | 608 |
| LLPS-Cis-0163 | Ciona savignyi | 62.2 | 6e-168 | 491 |
| LLPS-Drm-0812 | Drosophila melanogaster | 59.52 | 3e-140 | 423 |
| LLPS-Cii-0461 | Ciona intestinalis | 57.06 | 9e-60 | 201 |
| LLPS-Cae-0007 | Caenorhabditis elegans | 34.08 | 1e-61 | 214 |
| LLPS-Php-0002 | Physcomitrella patens | 32.06 | 1e-37 | 150 |
| LLPS-Amt-1087 | Amborella trichopoda | 31.8 | 4e-33 | 137 |
| LLPS-Mae-1919 | Manihot esculenta | 31.44 | 8e-34 | 136 |
| LLPS-Hea-2503 | Helianthus annuus | 31.27 | 1e-31 | 132 |
| LLPS-Nia-2099 | Nicotiana attenuata | 31.1 | 2e-32 | 135 |
| LLPS-Coc-0119 | Corchorus capsularis | 30.65 | 1e-28 | 124 |
| LLPS-Gor-1044 | Gossypium raimondii | 30.65 | 1e-29 | 127 |
| LLPS-Thc-1271 | Theobroma cacao | 30.36 | 4e-30 | 128 |
| LLPS-Phv-1414 | Phaseolus vulgaris | 30.27 | 1e-27 | 121 |
| LLPS-Viv-0120 | Vitis vinifera | 30.25 | 3e-31 | 132 |
| LLPS-Orp-0626 | Oryza punctata | 30.21 | 1e-25 | 114 |
| LLPS-Sem-0342 | Selaginella moellendorffii | 30.16 | 9e-33 | 135 |
| LLPS-Mua-1133 | Musa acuminata | 30.03 | 5e-26 | 117 |
| LLPS-Zem-1702 | Zea mays | 29.91 | 1e-25 | 115 |
| LLPS-Cus-0789 | Cucumis sativus | 29.74 | 6e-29 | 125 |
| LLPS-Glm-0257 | Glycine max | 29.74 | 5e-27 | 119 |
| LLPS-Sei-0534 | Setaria italica | 29.71 | 6e-26 | 116 |
| LLPS-Ori-1016 | Oryza indica | 29.68 | 2e-25 | 114 |
| LLPS-Org-0227 | Oryza glaberrima | 29.68 | 5e-26 | 114 |
| LLPS-Ors-0128 | Oryza sativa | 29.68 | 5e-26 | 114 |
| LLPS-Via-0087 | Vigna angularis | 29.67 | 7e-28 | 119 |
| LLPS-Hov-0542 | Hordeum vulgare | 29.52 | 4e-23 | 107 |
| LLPS-Brn-0639 | Brassica napus | 29.46 | 1e-27 | 120 |
| LLPS-Brr-0287 | Brassica rapa | 29.43 | 1e-27 | 120 |
| LLPS-Tra-2419 | Triticum aestivum | 29.31 | 2e-22 | 105 |
| LLPS-Chr-0948 | Chlamydomonas reinhardtii | 29.26 | 2e-18 | 92.8 |
| LLPS-Brd-1608 | Brachypodium distachyon | 29.18 | 1e-26 | 117 |
| LLPS-Art-0642 | Arabidopsis thaliana | 29.12 | 5e-26 | 115 |
| LLPS-Sob-0526 | Sorghum bicolor | 28.98 | 1e-23 | 108 |
| LLPS-Vir-0087 | Vigna radiata | 28.81 | 2e-25 | 112 |
| LLPS-Met-0314 | Medicago truncatula | 28.7 | 6e-25 | 113 |
| LLPS-Prp-0873 | Prunus persica | 28.61 | 2e-27 | 120 |
| LLPS-Arl-0177 | Arabidopsis lyrata | 28.45 | 3e-26 | 116 |
| LLPS-Lep-1677 | Leersia perrieri | 28.31 | 4e-22 | 103 |
| LLPS-Gas-0418 | Galdieria sulphuraria | 28.28 | 4e-28 | 120 |
| LLPS-Yal-0559 | Yarrowia lipolytica | 27.86 | 1e-11 | 70.9 |
| LLPS-Pot-2018 | Populus trichocarpa | 27.76 | 8e-27 | 119 |
| LLPS-Dac-0229 | Daucus carota | 27.62 | 5e-24 | 110 |
| LLPS-Bro-0471 | Brassica oleracea | 27.35 | 1e-24 | 112 |
| LLPS-Orgl-1409 | Oryza glumaepatula | 27.25 | 4e-20 | 98.2 |
| LLPS-Orni-0297 | Oryza nivara | 27.23 | 3e-21 | 102 |
| LLPS-Orm-0974 | Oryza meridionalis | 26.98 | 2e-21 | 102 |
| LLPS-Orr-0810 | Oryza rufipogon | 26.98 | 2e-21 | 102 |
| LLPS-Sol-2176 | Solanum lycopersicum | 26.74 | 2e-10 | 67.4 |
| LLPS-Orb-1549 | Oryza barthii | 25.58 | 6e-16 | 84.0 |
| LLPS-Abg-1027 | Absidia glauca | 25.43 | 2e-17 | 87.8 |
| LLPS-Scj-0285 | Schizosaccharomyces japonicus | 24.35 | 1e-09 | 64.7 |
| LLPS-Scp-1031 | Schizosaccharomyces pombe | 24.0 | 2e-09 | 64.3 |
| LLPS-Tru-1160 | Triticum urartu | 23.51 | 8e-12 | 72.0 |