• Disorder▼
  • Domain▼
  • PTM▼
  • Variation▼
  • Mutation▼
  • Interaction▼
  • Disease▼
  • Drug▼
  • Physicochemical▼
  • Function▼
  • Proteomics▼
  • Structure▼
  • Localization▼
  • Expression▼
  • Element▼
  • Methylation▼

LLPS-Bot-1619
ATG3

Integrated Annotations

▼ OVERVIEW

Status: Unreviewed
Protein Name: Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like
Gene Name: ATG3
Ensembl Gene: ENSBTAG00000009084.4
Ensembl Protein: ENSBTAP00000011976.3
Organism: Bos taurus
Taxa ID: 9913
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Stress granulePredicted from orthologs(View)

▼ FUNCTION

E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (By similarity).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MQNVINTVKG  KALEVAEYLT  PVLKESKFKE  TGVITPEEFV  AAGDHLVHHC  PTWQWATGEE  60
61    LKVKAYLPSG  KQFLVTKNVP  CYKRCKQMEY  SDELEAIIEE  DDGDGGWVDT  YHNTGIAGIT  120
121   EAVKEITLES  KDSIKLQDCS  ALCEEEEEED  EGEAADMEEY  EESGLLETDE  ATLDTRKIVE  180
181   ACKAKTDAGG  EDAILQTRTY  DLYITYDKYY  QTPRLWLFGY  DEQRQPLTVE  HMYEDISQDH  240
241   VKKTVTIENH  PHLPPPPMCS  VHPCRHAEVM  KKIIETVAEG  GGELGVHMYL  LIFLKFVQAV  300
301   IPTIEYDYTR  HFTM  314
Nucleotide CDS Sequence (FASTA)
1     ATGCAGAATG  TGATTAACAC  GGTGAAGGGA  AAGGCACTGG  AAGTGGCTGA  GTACCTGACC  60
61    CCGGTCCTCA  AGGAATCAAA  GTTTAAGGAA  ACAGGAGTTA  TCACCCCAGA  AGAGTTTGTG  120
121   GCAGCTGGAG  ATCACTTAGT  CCACCACTGT  CCAACATGGC  AATGGGCTAC  AGGGGAAGAA  180
181   TTGAAAGTGA  AGGCTTACCT  ACCATCAGGC  AAACAATTTT  TGGTCACCAA  AAATGTTCCA  240
241   TGCTATAAGC  GGTGTAAACA  GATGGAATAT  TCAGATGAAT  TGGAAGCTAT  CATTGAAGAA  300
301   GATGATGGCG  ATGGGGGATG  GGTAGATACT  TATCATAATA  CAGGTATTGC  AGGAATAACC  360
361   GAAGCAGTTA  AGGAGATTAC  ACTGGAAAGC  AAGGACAGTA  TAAAACTCCA  AGATTGCTCA  420
421   GCTTTATGTG  AGGAGGAGGA  AGAGGAAGAT  GAAGGAGAAG  CTGCAGATAT  GGAAGAATAT  480
481   GAAGAGAGTG  GATTGTTGGA  AACAGATGAG  GCTACTCTAG  ATACAAGGAA  AATAGTAGAA  540
541   GCTTGTAAAG  CTAAAACTGA  TGCTGGAGGT  GAGGATGCTA  TTTTGCAAAC  CAGAACTTAT  600
601   GACCTTTACA  TCACTTATGA  TAAATATTAC  CAGACACCAC  GACTATGGTT  GTTCGGCTAT  660
661   GATGAGCAAC  GGCAGCCTTT  AACAGTTGAG  CACATGTACG  AAGACATCAG  TCAAGATCAT  720
721   GTGAAGAAAA  CAGTGACCAT  TGAAAATCAC  CCTCATCTCC  CACCACCTCC  TATGTGTTCA  780
781   GTTCACCCAT  GCAGGCATGC  TGAGGTGATG  AAGAAAATCA  TTGAGACTGT  TGCAGAAGGA  840
841   GGGGGAGAAC  TTGGTGTTCA  TATGTATCTT  CTAATTTTCT  TGAAATTTGT  ACAAGCTGTC  900
901   ATTCCAACAA  TAGAATATGA  CTACACAAGA  CACTTCACGA  TGTAA  945

▼ KEYWORD

ID
Family
Acetylation
Autophagy
Complete proteome
Cytoplasm
Isopeptide bond
Protein transport
Reference proteome
Transferase
Transport
Ubl conjugation
Ubl conjugation pathway

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasmic ubiquitin ligase complex
Cellular Component
Cytosol
Molecular Function
Atg12 transferase activity
Molecular Function
Atg8 ligase activity
Molecular Function
Enzyme binding
Biological Process
Autophagosome assembly
Biological Process
Autophagy of mitochondrion
Biological Process
Mitochondrial fragmentation involved in apoptotic process
Biological Process
Negative regulation of phagocytosis
Biological Process
Protein transport
Biological Process
Protein ubiquitination
Biological Process
Regulation of cilium assembly

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Urm-2168Ursus maritimus100.02e-108 319
LLPS-Pap-1241Pan paniscus100.02e-108 319
LLPS-Ova-3310Ovis aries99.680.0 605
LLPS-Tut-1244Tursiops truncatus99.660.0 557
LLPS-Sus-1721Sus scrofa99.360.0 602
LLPS-Cas-2908Carlito syrichta99.040.0 600
LLPS-Ict-0976Ictidomys tridecemlineatus99.040.0 600
LLPS-Fud-1709Fukomys damarensis98.730.0 595
LLPS-Orc-1173Oryctolagus cuniculus98.410.0 598
LLPS-Caj-0866Callithrix jacchus98.410.0 598
LLPS-Mup-1880Mustela putorius furo98.410.0 600
LLPS-Aim-0162Ailuropoda melanoleuca98.410.0 596
LLPS-Caf-3979Canis familiaris98.390.0 590
LLPS-Hos-0595Homo sapiens98.090.0 598
LLPS-Pat-2811Pan troglodytes98.090.0 598
LLPS-Man-2831Macaca nemestrina98.090.0 596
LLPS-Mam-0574Macaca mulatta98.090.0 596
LLPS-Gog-0868Gorilla gorilla98.090.0 598
LLPS-Mum-1504Mus musculus98.090.0 598
LLPS-Maf-0417Macaca fascicularis98.090.0 596
LLPS-Chs-1844Chlorocebus sabaeus98.090.0 596
LLPS-Cea-1781Cercocebus atys98.090.0 596
LLPS-Nol-1835Nomascus leucogenys97.920.0 548
LLPS-Otg-0905Otolemur garnettii97.780.0 595
LLPS-Rhb-0022Rhinopithecus bieti97.770.0 594
LLPS-Mea-3005Mesocricetus auratus97.770.0 598
LLPS-Ran-2913Rattus norvegicus97.770.0 597
LLPS-Paa-1504Papio anubis97.770.0 595
LLPS-Mal-1000Mandrillus leucophaeus97.770.0 596
LLPS-Dio-1040Dipodomys ordii97.770.0 595
LLPS-Poa-1253Pongo abelii97.450.0 590
LLPS-Cap-1163Cavia porcellus97.160.0 590
LLPS-Sah-0348Sarcophilus harrisii95.540.0 581
LLPS-Myl-1276Myotis lucifugus95.30.0 542
LLPS-Ere-0829Erinaceus europaeus95.220.0 578
LLPS-Fec-1704Felis catus93.090.0 587
LLPS-Fia-2875Ficedula albicollis92.360.0 568
LLPS-Tag-1204Taeniopygia guttata92.360.0 566
LLPS-Meg-2851Meleagris gallopavo92.040.0 562
LLPS-Gaga-3720Gallus gallus92.040.0 562
LLPS-Anc-2239Anolis carolinensis91.110.0 555
LLPS-Ora-2394Ornithorhynchus anatinus89.666e-134 387
LLPS-Mod-1873Monodelphis domestica88.250.0 558
LLPS-Loa-1578Loxodonta africana88.050.0 552
LLPS-Pes-1510Pelodiscus sinensis87.240.0 510
LLPS-Pof-0675Poecilia formosa84.810.0 519
LLPS-Xet-1650Xenopus tropicalis84.760.0 526
LLPS-Aon-2165Aotus nancymaae84.542e-164 467
LLPS-Xim-1120Xiphophorus maculatus84.490.0 519
LLPS-Leo-0736Lepisosteus oculatus84.440.0 517
LLPS-Orn-1968Oreochromis niloticus84.080.0 516
LLPS-Scf-1344Scleropages formosus83.760.0 527
LLPS-Lac-0954Latimeria chalumnae83.440.0 539
LLPS-Eqc-1155Equus caballus82.870.0 577
LLPS-Icp-0296Ictalurus punctatus81.850.0 513
LLPS-Dar-1417Danio rerio79.810.0 525
LLPS-Asm-2819Astyanax mexicanus79.634e-175 495
LLPS-Tar-0432Takifugu rubripes79.432e-179 506
LLPS-Scm-1864Scophthalmus maximus77.741e-155 448
LLPS-Orl-2456Oryzias latipes77.711e-160 457
LLPS-Gaa-2836Gasterosteus aculeatus76.632e-174 494
LLPS-Anp-1880Anas platyrhynchos75.977e-150 431
LLPS-Ten-0044Tetraodon nigroviridis74.921e-165 471
LLPS-Cis-1271Ciona savignyi63.924e-136 395
LLPS-Cii-1410Ciona intestinalis63.385e-137 398
LLPS-Drm-0354Drosophila melanogaster62.242e-135 394
LLPS-Abg-0999Absidia glauca60.942e-49 167
LLPS-Asfu-0060Aspergillus fumigatus54.171e-1785.9
LLPS-Asf-1433Aspergillus flavus53.522e-1785.5
LLPS-Aso-0684Aspergillus oryzae53.522e-1785.5
LLPS-Usm-0971Ustilago maydis52.945e-2299.4
LLPS-Dos-0108Dothistroma septosporum52.51e-1580.5
LLPS-Asg-0262Ashbya gossypii51.434e-1577.8
LLPS-Asn-0643Aspergillus nidulans51.392e-1682.8
LLPS-Ast-0164Aspergillus terreus51.252e-1784.0
LLPS-Cae-0413Caenorhabditis elegans50.781e-98 300
LLPS-Mel-0651Melampsora laricipopulina49.471e-23 103
LLPS-Cus-0202Cucumis sativus47.289e-71 228
LLPS-Asc-0627Aspergillus clavatus47.192e-1682.8
LLPS-Brd-1174Brachypodium distachyon47.16e-73 234
LLPS-Beb-0754Beauveria bassiana47.062e-1785.5
LLPS-Yal-1076Yarrowia lipolytica46.885e-1990.1
LLPS-Gor-0747Gossypium raimondii46.83e-73 235
LLPS-Hea-0050Helianthus annuus46.786e-74 237
LLPS-Via-1057Vigna angularis46.782e-74 238
LLPS-Pot-1829Populus trichocarpa46.764e-75 239
LLPS-Tru-0608Triticum urartu46.769e-72 231
LLPS-Tra-1042Triticum aestivum46.769e-72 231
LLPS-Sob-1443Sorghum bicolor46.696e-73 234
LLPS-Art-2589Arabidopsis thaliana46.623e-74 238
LLPS-Coc-0452Corchorus capsularis46.287e-72 231
LLPS-Arl-1476Arabidopsis lyrata46.281e-73 236
LLPS-Dac-1895Daucus carota46.253e-74 238
LLPS-Hov-1235Hordeum vulgare46.082e-70 228
LLPS-Spr-1127Sporisorium reilianum45.876e-23 102
LLPS-Met-0667Medicago truncatula45.762e-72 233
LLPS-Orbr-1549Oryza brachyantha45.734e-71 229
LLPS-Mae-0741Manihot esculenta45.614e-72 232
LLPS-Phv-1363Phaseolus vulgaris45.611e-70 228
LLPS-Thc-0698Theobroma cacao45.615e-71 229
LLPS-Sot-0227Solanum tuberosum45.61e-75 241
LLPS-Glm-1092Glycine max45.362e-71 230
LLPS-Prp-1049Prunus persica45.214e-72 232
LLPS-Sol-0666Solanum lycopersicum44.977e-75 239
LLPS-Orm-0387Oryza meridionalis44.865e-73 234
LLPS-Gag-0777Gaeumannomyces graminis44.761e-1786.3
LLPS-Nia-0907Nicotiana attenuata44.748e-73 234
LLPS-Php-1923Physcomitrella patens44.744e-75 239
LLPS-Asni-1594Aspergillus niger44.663e-1887.4
LLPS-Amt-0706Amborella trichopoda44.282e-58 196
LLPS-Bro-1129Brassica oleracea44.272e-71 230
LLPS-Orgl-1837Oryza glumaepatula44.271e-55 192
LLPS-Ors-2271Oryza sativa44.181e-71 231
LLPS-Ori-1630Oryza indica44.181e-71 231
LLPS-Brr-0283Brassica rapa43.773e-70 227
LLPS-Brn-2921Brassica napus43.773e-70 227
LLPS-Org-1591Oryza glaberrima43.671e-73 236
LLPS-Mua-0053Musa acuminata43.653e-72 233
LLPS-Orp-0840Oryza punctata43.31e-57 197
LLPS-Zem-2104Zea mays43.138e-69 224
LLPS-Orb-1341Oryza barthii43.133e-70 227
LLPS-Osl-0739Ostreococcus lucimarinus42.778e-70 225
LLPS-Lep-0010Leersia perrieri42.533e-56 192
LLPS-Sem-0333Selaginella moellendorffii42.523e-67 219
LLPS-Orr-0536Oryza rufipogon42.339e-69 224
LLPS-Cogr-0100Colletotrichum graminicola42.162e-1887.8
LLPS-Orni-0417Oryza nivara42.153e-55 192
LLPS-Sei-0094Setaria italica41.893e-43 155
LLPS-Chr-1270Chlamydomonas reinhardtii40.974e-77 244
LLPS-Put-0748Puccinia triticina40.422e-55 191
LLPS-Crn-1021Cryptococcus neoformans39.671e-57 197
LLPS-Vir-0221Vigna radiata39.582e-57 199
LLPS-Lem-1098Leptosphaeria maculans38.646e-35 135
LLPS-Tum-1528Tuber melanosporum38.245e-1371.2
LLPS-Pytr-1101Pyrenophora triticirepentis37.552e-35 137
LLPS-Pyt-0811Pyrenophora teres37.551e-35 137
LLPS-Pug-0404Puccinia graminis37.05e-55 190
LLPS-Kop-0807Komagataella pastoris36.52e-50 176
LLPS-Scp-1104Schizosaccharomyces pombe36.245e-48 168
LLPS-Scc-0174Schizosaccharomyces cryophilus35.886e-47 166
LLPS-Zyt-0132Zymoseptoria tritici35.869e-35 135
LLPS-Trv-1333Trichoderma virens35.845e-39 146
LLPS-Trr-0934Trichoderma reesei35.481e-37 142
LLPS-Blg-0487Blumeria graminis35.092e-36 139
LLPS-Scj-1179Schizosaccharomyces japonicus34.83e-47 166
LLPS-Phn-0939Phaeosphaeria nodorum34.783e-33 130
LLPS-Miv-0504Microbotryum violaceum34.549e-57 194
LLPS-Sac-1269Saccharomyces cerevisiae34.264e-42 154
LLPS-Fus-1029Fusarium solani33.796e-39 146
LLPS-Fuo-0136Fusarium oxysporum33.228e-37 140
LLPS-Coo-1477Colletotrichum orbiculare33.222e-39 147
LLPS-Fuv-0105Fusarium verticillioides32.896e-38 143
LLPS-Nec-1012Neurospora crassa32.185e-37 140
LLPS-Mao-0761Magnaporthe oryzae32.091e-42 156
LLPS-Ved-0412Verticillium dahliae31.546e-43 157
LLPS-Nef-1174Neosartorya fischeri31.041e-40 150
LLPS-Cog-0277Colletotrichum gloeosporioides30.211e-41 153