LLPS-Asm-a924
Integrated Annotations
▼ OVERVIEW
| Status: | Unreviewed |
| Protein Name: | AP-2 complex subunit alpha |
| Ensembl Gene: | ENSAMXG00000002953.2 |
| Ensembl Protein: | ENSAMXP00000003083.2 |
| Organism: | Astyanax mexicanus |
| Taxa ID: | 7994 |
| LLPS Type: | Client |
▼ Classification
Condensates:
| Condensate | Evidence | Orthologs |
|---|---|---|
| Postsynaptic density | Predicted from orthologs | (View) |
▼ FUNCTION
| Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENSAMXT00000003083.2 | ENSAMXP00000003083.2 |
| UniProt | W5K672, W5K672_ASTMX | |
| Entrez | 103033785 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL DGYSKKKYVC 60 61 KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV LVNSNSDLIR LINNAIKNDL 120 121 SSRNPTYMNL ALHCIANVGS REMAEAFASE VPRILVAGDT MDSVKQSAAL CLLRLNRTSP 180 181 DLVPMGEWTS RVVHLLNDQH LGVVTAATSL ISTLAQKNPD DFKTSVSLAV ARLSRIVTSA 240 241 STDLQDYTYY FVAAPWLSVK LLRLLQCYPP PEEGAIRGRL TECLETILNK AQEPPKSKKV 300 301 QHSNAKNAVL FEAISLIIHH DSEPTLLVRA CNQLGQFLQH RETNLRYLAL ESMCTLASSE 360 361 FSHEAVKTHI ETVINSLKTE RDVSVRQRAV DLLYAMCDRS NAKQIVTEML SYLETADYSI 420 421 REEIVLKVAI LAEKYAVDYT WYVDTILNLI RIAGDYVSEE VWYRVIQIVI NRDDVQGYAA 480 481 KTVFEALQAP ACHENLVKVG GYILGEFGNL IAGDPRSSPL VQFHLLHSKF HLCSVPTRAL 540 541 LLSAYVKFIN LFPEVKGTIQ EVLRSDSQLR NADVELQQRA VEYLRLSCIA TTDILATVLE 600 601 EMPPFPERES SILAKLKKKK GSGTVSDIED SRKERNVNAN ANVTSSPSSD LLGLGNSSAV 660 661 NSASPPTSSH AASLLVDVFS ESLPVAALGS ASAHVIEENF SRFVCKNNGV LFESQLLQIG 720 721 LKSEFRQNLG RMYIFYGNKT STQFLNFSSS VVCPEPLTAD PVLDGGAQLQ QILNIECISD 780 781 FVEAPVLNIQ FRYGGSTQNI SVRLPVTLNK FFQPTEMTSQ DFFQRWKQLS PPQQEVQKIF 840 841 KAKHSMDTEI TKAKIIGFGT ALLDGVDPNP SNFVGAGVIH TKTIQVGCLL RLEPNTQAQV 900 901 STAALGLDDL HFSESLASLF HTHL 924 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGTGTTTTA CAGGTAAAAG TAAAGAGGCT GAAATCAAAA GAATAAACAA AGAGTTGGCC 60 61 AATATTCGCT CCAAGTTTAA AGGAGATAAA GCGCTGGATG GCTACAGTAA GAAGAAATAT 120 121 GTCTGTAAAC TGCTCTTCAT TTTTCTGCTG GGTCACGACA TCGATTTCGG CCACATGGAG 180 181 GCAGTCAACC TCCTCAGCTC CAACAAATAC ACAGAGAAAC AGATCGGCTA CCTGTTCATC 240 241 TCGGTGCTGG TGAACAGTAA CAGCGATCTG ATCAGACTGA TCAACAACGC CATAAAGAAT 300 301 GACCTGTCCA GCAGAAACCC CACCTACATG AACCTGGCCC TGCACTGCAT TGCAAACGTG 360 361 GGCAGCAGGG AGATGGCAGA GGCATTTGCA TCAGAAGTAC CACGAATCCT GGTGGCGGGT 420 421 GACACCATGG ACAGTGTGAA GCAGAGTGCA GCACTGTGTT TATTACGGCT CAACCGGACC 480 481 TGTCCTGACC TCGTCCCAAT GGGAGAGTGG ACTTCACGTG TCGTTCACCT TCTCAACGAT 540 541 CAGCACCTGG GAGTGGTGAC CGCTGCAACC AGTCTGATCA GCACTCTGGC TCAGAAAAAC 600 601 CCAGACGACT TCAAGACCTC TGTTTCTCTG GCTGTGGCCA GACTCAGCCG GATCGTCACA 660 661 TCAGCCTCCA CTGACCTACA GGACTACACA TATTATTTTG TTGCTGCACC GTGGCTGTCA 720 721 GTCAAACTGC TCCGCCTTCT GCAGTGTTAC CCACCACCAG AGGAGGGAGC GATTCGAGGA 780 781 CGCCTGACTG AATGTTTGGA AACAATCCTG AACAAAGCGC AAGAGCCTCC AAAGTCCAAA 840 841 AAAGTCCAGC ACTCCAATGC CAAGAATGCA GTTCTGTTCG AAGCCATCAG TCTCATCATA 900 901 CACCATGACA GTGAACCGAC GCTGCTGGTC CGCGCCTGTA ATCAGCTGGG TCAGTTTTTG 960 961 CAGCACAGAG AGACTAATCT GCGCTACCTG GCCTTGGAGA GCATGTGCAC CCTCGCCAGT 1020 1021 TCAGAGTTCT CCCATGAAGC AGTTAAAACG CACATCGAGA CTGTAATCAA CTCTCTTAAG 1080 1081 ACTGAGCGGG ATGTGAGTGT GAGGCAGCGT GCTGTTGACC TGCTCTACGC CATGTGTGAT 1140 1141 CGCAGCAACG CCAAACAGAT CGTCACAGAG ATGCTCAGTT ACTTAGAAAC AGCCGACTAC 1200 1201 TCCATCCGAG AGGAAATCGT TTGTGTTTGT GTTTTATTCC TGTCAAACCT TTACTGGACT 1260 1261 GTTCTTTCAT CACTAAGACT AAATAGCAGC TGTGGCTCGA CTCCAAGCTT TACTGGCTTT 1320 1321 CACAGCGCCT TACTATTAAA TGTACTATTA AATCTGAACA GTTTGTTGCA TTTTTCATTG 1380 1381 TGCATAACAT TCAGTCATAT ACCTGTTCCA TCATGTCTAC TAGATCTTTT GGGGGTGTTT 1440 1441 ATCAAGAATG TCTTCAGTAT CTTCTGTCTC TCTCTCTCTC ACTTCATCAG TCCACTTGTT 1500 1501 CAGTTCCACC TGCTCCACTC CAAGTTCCAC CTGTGTTCGG TTCCCACTCG CGCTCTGCTG 1560 1561 CTCTCGGCGT ACGTTAAGTT CATCAACCTG TTCCCGGAAG TGAAAGGTAC CATACAGGAA 1620 1621 GTGCTGCGCT CGGATAGTCA GCTGAGGAAT GCAGATGTGG AGCTTCAGCA GCGAGCTGTG 1680 1681 GAGTACCTCC GCCTGAGCTG CATCGCCACC ACGGACATAC TGGCTACAGT TCTGGAGGAG 1740 1741 ATGCCTCCTT TCCCCGAGCG AGAGTCTTCC ATCTTGGCGA AACTGAAGAA GAAGAAAGGT 1800 1801 TCTGGAACTG TGTCTGATAT TGAAGACAGT CGCAAAGAGC GCAACGTCAA TGCCAACGCC 1860 1861 AACGTCAACG GCAGCAGCGA TCACACCATC ACCAACACAA GCGCAAAGCC TCCTCCCTCT 1920 1921 TTTGCTTGTG ATCTATTGGC TACAGCTCCA CCCCCCACCA ACAGGCCACG CCCTATCCCT 1980 1981 TCCTCCTCCA TGCTGCACAC GCCCCTCACC CCTTTATCAC CGAAGGCTTA TACACCTCAG 2040 2041 CCTGTTACGC CAACTGTCCC CTTTTATCAG GTTACTTCTT CCCCGTCGTC AGATCTTCTG 2100 2101 GGCCTGGGGA ACAGCTCAGC AGTAAACTCA GCTAGTCCTC CAACCTCCTC ACACGCAGCC 2160 2161 AGCCTGCTGG TGGATGTGTT CTCAGAAAGC CTGCCTGTAG CAGCTTTGGG TTCTGCCTCT 2220 2221 GCACACGTCA TAGAGGAAAA CTTCTCAAGG TTCGTCTGCA AGAATAACGG AGTGTTGTTT 2280 2281 GAGAGTCAGC TGCTGCAGAT TGGCCTGAAG TCTGAGTTCA GACAGAACCT GGGTCGCATG 2340 2341 TACATTTTCT ACGGAAACAA AACGTCAACG CAGTTCCTGA ATTTTTCATC CTCTGTGGTC 2400 2401 TGTCCCGAAC CACTCAGCTC CCATCTGAAC ATTCACATGA AAGCTGCTGA CCCTGTGCTG 2460 2461 GACGGAGGAG CTCAGCTTCA GCAGATCCTG AACATCGAGT GCATATCTGA CTTTGTGGAA 2520 2521 GCACCAGTGC TTAATATTCA ATTCAGGTAC GGAGGCAGCA CACAGAATAT CTCCGTCAGG 2580 2581 CTTCCCGTCA CCCTCAACAA GTTCTTCCAG CCCACAGAGA TGACGTCTCA GGACTTCTTC 2640 2641 CAGCGCTGGA AGCAGCTCAG CGCTCCCCAG CAGGAGGTGC AGAAGATCTT CAAAGCCAAG 2700 2701 CATTCTATGG ACACAGAAAT CACGAAAGCT AAGATCATCG GGTTTGGAAC AGCTCTGCTG 2760 2761 GATGGAGTAG ACCCAAATCC TTCAAACTTT GTGGGTGCCG GAGTGATCCA CACCAAGACT 2820 2821 ATACAGGTTG GCTGCCTCCT GAGACTAGAG CCCAACACTC AGGCACAGAT GTACCGACTG 2880 2881 ACTCTAAGGA CAAGCCGCGA GTCGGTGACT CAGAGACTGA GTAATCTTCT GTCGGATCAG 2940 2941 TTCTGA 2946 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | AP-2 adaptor complex | |
Molecular Function | Clathrin adaptor activity | |
Biological Process | Clathrin-dependent endocytosis | |
Biological Process | Intracellular protein transport |