LLPS-Art-1111
HTR8

▼ OVERVIEW

Status: Reviewed
Protein Name: Histone H3.3; Histone H3.2
Gene Name: HTR8, At4g40030, T5J17.200, At4g40040, T5J17.210, At5g10980, T30N20_250, T5K6.6
Ensembl Gene: AT4G40030
Ensembl Protein: AT4G40030.4
Organism: Arabidopsis thaliana
Taxa ID: 3702
LLPS Type: Client
PDB: 4PL6 (A, C) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Nucleolus
"...Comparative analysis revealed that 562 proteins were consistently identified in both experimental data sets."
3 weeks-old Arabidopsis thaliana plants29104584
Nucleolus
"...We identified 1602 proteins in the nucleolar and 2544 proteins in the nuclear fraction with an overlap of 1429 proteins."
Arabidopsis thaliana cells26980300

▼ FUNCTION

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MFFLLLSPRS  DFTTIEFRVL  SHSSLKIKMA  RTKQTARKST  GGKAPRKQLA  TKAARKSAPT  60
61    TGGVKKPHRY  RPGTVALREI  RKYQKSTELL  IRKLPFQRLV  REIAQDFKTD  LRFQSHAVLA  120
121   LQEAAEAYLV  GLFEDTNLCA  IHAKRVTIMP  KDIQLARRIR  GERA  164
Nucleotide CDS Sequence (FASTA)
1     ATGTTTTTTC  TGTTACTGTC  TCCGCGATCT  GATTTTACGA  CAATAGAGTT  TCGGGTTTTG  60
61    TCCCATTCCA  GTTTGAAAAT  AAAGATGGCT  CGTACCAAGC  AAACCGCTCG  TAAGTCCACC  120
121   GGAGGTAAAG  CTCCAAGGAA  GCAACTTGCT  ACTAAGGCTG  CTCGTAAATC  TGCACCAACT  180
181   ACTGGTGGAG  TCAAGAAACC  ACATCGTTAC  CGTCCTGGAA  CTGTTGCTCT  CCGTGAAATC  240
241   CGTAAGTACC  AGAAGAGTAC  TGAGTTGCTT  ATCAGGAAAC  TGCCATTTCA  GAGGCTAGTC  300
301   CGTGAGATTG  CTCAAGATTT  CAAGACTGAT  TTGCGTTTCC  AGAGCCATGC  TGTCTTAGCT  360
361   CTCCAGGAAG  CTGCAGAAGC  ATATCTTGTT  GGTCTCTTTG  AAGACACTAA  CCTTTGTGCC  420
421   ATTCATGCCA  AGCGTGTGAC  CATAATGCCC  AAAGACATTC  AGCTCGCTCG  TCGTATCAGA  480
481   GGTGAACGCG  CTTAA  495

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Alternative splicing
Chromosome
Complete proteome
DNA-binding
Methylation
Nucleosome core
Nucleus
Phosphoprotein
Reference proteome

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Nucleolus
Cellular Component
Nucleosome
Cellular Component
Nucleus
Cellular Component
RDNA protrusion
Molecular Function
Nucleosomal DNA binding
Molecular Function
Protein heterodimerization activity

▼ KEGG


▼ ANNOTATION

Disorder
IUPred2A
Physicochemical
Compute pI/MwAAindex
Function
CGDBPIRSF
Structure
PDB
Localization
COMPARTMENTSNLSdb

▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Zem-1042Zea mays100.02e-93 275
LLPS-Org-1713Oryza glaberrima100.02e-93 275
LLPS-Tru-0846Triticum urartu100.01e-93 277
LLPS-Bro-0754Brassica oleracea100.02e-93 275
LLPS-Prp-0855Prunus persica100.02e-93 275
LLPS-Orr-0917Oryza rufipogon100.02e-93 275
LLPS-Mae-0634Manihot esculenta100.02e-93 275
LLPS-Phv-2417Phaseolus vulgaris100.02e-93 275
LLPS-Nia-0321Nicotiana attenuata100.02e-93 275
LLPS-Orbr-2149Oryza brachyantha100.01e-91 277
LLPS-Pot-2089Populus trichocarpa100.02e-93 275
LLPS-Ors-2104Oryza sativa100.02e-93 275
LLPS-Arl-2946Arabidopsis lyrata100.02e-93 275
LLPS-Sei-1272Setaria italica100.02e-93 275
LLPS-Cus-0860Cucumis sativus100.02e-93 275
LLPS-Hov-0876Hordeum vulgare100.02e-93 275
LLPS-Met-0228Medicago truncatula100.02e-93 275
LLPS-Brd-1732Brachypodium distachyon100.02e-93 275
LLPS-Tra-0631Triticum aestivum100.02e-93 275
LLPS-Sot-1788Solanum tuberosum100.02e-93 275
LLPS-Orgl-0421Oryza glumaepatula100.02e-93 275
LLPS-Thc-0291Theobroma cacao100.02e-93 275
LLPS-Orni-2352Oryza nivara100.02e-93 275
LLPS-Dac-2250Daucus carota100.02e-93 275
LLPS-Php-1728Physcomitrella patens100.02e-93 275
LLPS-Brn-3269Brassica napus100.02e-93 275
LLPS-Orb-0081Oryza barthii100.02e-93 275
LLPS-Ori-1646Oryza indica100.02e-93 275
LLPS-Sem-0633Selaginella moellendorffii100.02e-93 275
LLPS-Lep-0498Leersia perrieri99.283e-91 275
LLPS-Viv-2271Vitis vinifera99.271e-93 275
LLPS-Glm-0630Glycine max99.271e-92 274
LLPS-Hea-0198Helianthus annuus98.551e-93 275
LLPS-Osl-0104Ostreococcus lucimarinus98.537e-92 270
LLPS-Amt-0494Amborella trichopoda98.532e-92 272
LLPS-Orm-0070Oryza meridionalis97.064e-90 266
LLPS-Coc-0989Corchorus capsularis97.064e-90 266
LLPS-Sob-1818Sorghum bicolor97.064e-90 266
LLPS-Orp-1274Oryza punctata97.064e-90 266
LLPS-Via-0879Vigna angularis97.065e-90 266
LLPS-Sol-1065Solanum lycopersicum97.064e-90 266
LLPS-Ora-1963Ornithorhynchus anatinus96.353e-91 269
LLPS-Ict-1339Ictidomys tridecemlineatus96.321e-90 267
LLPS-Nol-0002Nomascus leucogenys96.323e-90 266
LLPS-Anc-1452Anolis carolinensis96.321e-90 267
LLPS-Tag-0780Taeniopygia guttata96.321e-90 267
LLPS-Cas-0046Carlito syrichta96.323e-90 266
LLPS-Gaga-3013Gallus gallus96.321e-90 267
LLPS-Xet-3469Xenopus tropicalis96.321e-90 267
LLPS-Mam-4245Macaca mulatta96.323e-90 266
LLPS-Ten-3696Tetraodon nigroviridis96.321e-90 267
LLPS-Caf-0616Canis familiaris96.321e-90 267
LLPS-Poa-0725Pongo abelii96.323e-90 266
LLPS-Eqc-1180Equus caballus96.323e-90 266
LLPS-Cap-1596Cavia porcellus96.323e-90 266
LLPS-Fia-3109Ficedula albicollis96.321e-90 267
LLPS-Orc-2673Oryctolagus cuniculus96.321e-90 267
LLPS-Rhb-0859Rhinopithecus bieti96.323e-90 266
LLPS-Otg-0868Otolemur garnettii96.323e-90 266
LLPS-Pes-3239Pelodiscus sinensis96.321e-90 267
LLPS-Man-0003Macaca nemestrina96.321e-90 267
LLPS-Loa-1947Loxodonta africana96.323e-90 266
LLPS-Anp-0891Anas platyrhynchos96.321e-90 267
LLPS-Urm-0460Ursus maritimus96.323e-90 266
LLPS-Pap-2305Pan paniscus96.323e-90 266
LLPS-Pat-0695Pan troglodytes96.323e-90 266
LLPS-Pof-2981Poecilia formosa96.321e-90 267
LLPS-Dar-3826Danio rerio96.321e-90 267
LLPS-Hos-0101Homo sapiens96.323e-90 266
LLPS-Meg-0970Meleagris gallopavo96.321e-90 267
LLPS-Fec-1371Felis catus96.321e-90 267
LLPS-Mod-2272Monodelphis domestica96.321e-90 267
LLPS-Mal-0380Mandrillus leucophaeus96.321e-90 267
LLPS-Paa-3205Papio anubis96.323e-90 266
LLPS-Myl-0904Myotis lucifugus96.323e-90 266
LLPS-Ova-0036Ovis aries96.323e-90 266
LLPS-Aim-0597Ailuropoda melanoleuca96.323e-90 266
LLPS-Ran-2683Rattus norvegicus96.321e-90 267
LLPS-Ere-0983Erinaceus europaeus96.321e-90 267
LLPS-Dio-3132Dipodomys ordii96.321e-90 267
LLPS-Bot-1053Bos taurus96.321e-90 267
LLPS-Drm-0851Drosophila melanogaster96.321e-90 267
LLPS-Tut-1840Tursiops truncatus96.323e-90 266
LLPS-Fud-0153Fukomys damarensis96.321e-90 267
LLPS-Cae-1905Caenorhabditis elegans96.326e-90 265
LLPS-Icp-0990Ictalurus punctatus96.321e-90 267
LLPS-Caj-2784Callithrix jacchus96.323e-90 266
LLPS-Gog-3492Gorilla gorilla96.321e-90 267
LLPS-Orl-0115Oryzias latipes96.321e-90 267
LLPS-Cea-2960Cercocebus atys96.323e-90 266
LLPS-Aon-1026Aotus nancymaae96.321e-90 267
LLPS-Sus-2818Sus scrofa96.323e-90 266
LLPS-Mea-0428Mesocricetus auratus96.321e-90 267
LLPS-Mum-0749Mus musculus96.323e-90 266
LLPS-Maf-1171Macaca fascicularis96.321e-90 267
LLPS-Chs-2698Chlorocebus sabaeus96.321e-90 267
LLPS-Sah-2481Sarcophilus harrisii95.656e-91 268
LLPS-Gaa-3970Gasterosteus aculeatus95.622e-90 267
LLPS-Tar-1562Takifugu rubripes95.629e-91 268
LLPS-Cis-1993Ciona savignyi95.623e-90 266
LLPS-Orn-1719Oreochromis niloticus95.592e-89 264
LLPS-Lac-2482Latimeria chalumnae95.592e-89 264
LLPS-Scm-0925Scophthalmus maximus95.592e-89 264
LLPS-Mup-0402Mustela putorius furo95.592e-89 264
LLPS-Chc-0479Chondrus crispus95.599e-90 265
LLPS-Scf-0248Scleropages formosus95.592e-89 264
LLPS-Leo-1949Lepisosteus oculatus95.592e-89 264
LLPS-Gor-0132Gossypium raimondii93.332e-93 275