LLPS-Art-0550
MED36A
▼ OVERVIEW
| Status: | Reviewed |
| Protein Name: | Mediator of RNA polymerase II transcription subunit 36a; Fibrillarin-like protein 2; Histone-glutamine methyltransferase; rRNA 2'-O-methyltransferase fibrillarin 2 |
| Gene Name: | MED36A, FIB2, FLP, MED36_1, At4g25630, L73G19.10 |
| Ensembl Gene: | AT4G25630 |
| Ensembl Protein: | AT4G25630.1 |
| Organism: | Arabidopsis thaliana |
| Taxa ID: | 3702 |
| LLPS Type: | Client |
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|---|---|---|
| Nucleolus | "...We identified 1602 proteins in the nucleolar and 2544 proteins in the nuclear fraction with an overlap of 1429 proteins." | Arabidopsis thaliana cells | 26980300 |
| Nucleolus | "...Comparative analysis revealed that 562 proteins were consistently identified in both experimental data sets." | 3 weeks-old Arabidopsis thaliana plants | 29104584 |
| Nucleolus | "...Here, we describe the first proteomic analysis of plant nucleoli, in which we have identified 217 proteins." | Protoplasts | 15496452 |
| Cajal body, P-body, Stress granule | Predicted from orthologs | N/A | (View) |
▼ FUNCTION
| Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | AT4G25630.1 | AT4G25630.1 |
| UniProt | Q94AH9, MD36A_ARATH, Q9SZZ1 | |
| GeneBank | AY142647, AL161563, AF233444, AY046027, AF267171, AY084608, CP002687, AL050400 | AAN13105.1, AAG10104.1, AAK76701.1, AAG10153.1, AEE85085.1, CAB81373.1, AAM61172.1, CAB43694.1 |
| RefSeq | NM_118695.4 | NP_567724.1 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MRPPLTGSGG GFSGGRGRGG YSGGRGDGGF SGGRGGGGRG GGRGFSDRGG RGRGRGPPRG 60 61 GARGGRGPAG RGGMKGGSKV IVEPHRHAGV FIAKGKEDAL VTKNLVPGEA VYNEKRISVQ 120 121 NEDGTKTEYR VWNPFRSKLA AAILGGVDNI WIKPGAKVLY LGAASGTTVS HVSDLVGPEG 180 181 CVYAVEFSHR SGRDLVNMAK KRTNVIPIIE DARHPAKYRM LVGMVDVIFS DVAQPDQARI 240 241 LALNASYFLK SGGHFVISIK ANCIDSTVPA EAVFQTEVKK LQQEQFKPAE QVTLEPFERD 300 301 HACVVGGYRM PKKPKAATAA 320 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGAGACCTC CTCTAACTGG AAGTGGTGGT GGGTTCAGTG GTGGAAGAGG GCGTGGTGGA 60 61 TACAGTGGTG GTAGAGGTGA CGGTGGATTC AGTGGTGGTC GAGGTGGCGG TGGTAGAGGA 120 121 GGAGGAAGAG GTTTCAGCGA CCGTGGTGGT CGCGGCAGAG GAAGAGGACC ACCACGTGGT 180 181 GGTGCTCGCG GCGGCAGAGG ACCAGCTGGC CGTGGAGGCA TGAAAGGAGG AAGCAAAGTG 240 241 ATTGTGGAAC CTCACAGACA CGCAGGAGTG TTCATTGCAA AGGGTAAAGA AGATGCCCTT 300 301 GTTACCAAGA ATTTGGTTCC TGGTGAAGCT GTCTACAACG AGAAGAGAAT CTCTGTTCAG 360 361 AACGAAGATG GAACCAAGAC TGAATACAGA GTTTGGAATC CTTTCCGTTC GAAGTTGGCT 420 421 GCTGCTATTC TTGGTGGTGT TGATAACATC TGGATTAAAC CTGGTGCTAA AGTTCTATAC 480 481 CTTGGTGCTG CTTCTGGAAC CACAGTCTCT CATGTGTCTG ATCTTGTTGG CCCTGAGGGG 540 541 TGTGTGTACG CGGTTGAGTT TTCTCATAGA AGTGGTAGAG ATTTGGTGAA CATGGCAAAG 600 601 AAGAGAACCA ATGTTATTCC AATCATCGAG GATGCTAGAC ACCCAGCTAA ATACAGAATG 660 661 CTTGTAGGCA TGGTTGATGT TATCTTCTCT GATGTTGCTC AGCCAGATCA GGCTAGGATC 720 721 TTGGCTTTGA ATGCATCATA CTTCCTCAAG TCAGGAGGAC ACTTTGTGAT CTCAATAAAG 780 781 GCAAACTGTA TCGACTCCAC CGTTCCAGCA GAAGCTGTGT TCCAGACTGA AGTGAAGAAG 840 841 CTTCAACAGG AGCAATTCAA ACCAGCTGAG CAAGTGACGC TTGAGCCATT TGAGCGTGAC 900 901 CATGCATGTG TCGTTGGTGG CTACCGTATG CCTAAGAAGC CAAAGGCTGC TACTGCTGCT 960 961 TAG 963 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Box C/D snoRNP complex | |
Cellular Component | Cajal body | |
Cellular Component | Mediator complex | |
Cellular Component | Nucleolus | |
Cellular Component | Small-subunit processome | |
Molecular Function | Histone-glutamine methyltransferase activity | |
Molecular Function | Ribonuclease activity | |
Molecular Function | RNA binding | |
Molecular Function | RRNA methyltransferase activity | |
Molecular Function | SnoRNA binding | |
Biological Process | Box C/D snoRNA 3'-end processing | |
Biological Process | Histone glutamine methylation | |
Biological Process | RRNA methylation |
▼ ANNOTATION
| Disorder | ||||
| IUPred2A | ||||
| PTM | ||||
| PLMD | ||||
| Physicochemical | ||||
| Compute pI/Mw | AAindex | |||
| Localization | ||||
| COMPARTMENTS | NLSdb | |||
| Expression | ||||
| ArrayExpress | ||||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Arl-0861 | Arabidopsis lyrata | 98.38 | 5e-178 | 503 |
| LLPS-Brn-1263 | Brassica napus | 95.76 | 5e-166 | 472 |
| LLPS-Bro-0401 | Brassica oleracea | 95.76 | 7e-166 | 471 |
| LLPS-Gor-1278 | Gossypium raimondii | 95.02 | 4e-168 | 477 |
| LLPS-Brr-0099 | Brassica rapa | 94.74 | 4e-172 | 487 |
| LLPS-Coc-1282 | Corchorus capsularis | 94.54 | 7e-166 | 471 |
| LLPS-Sol-0500 | Solanum lycopersicum | 93.75 | 8e-164 | 466 |
| LLPS-Mae-1318 | Manihot esculenta | 93.36 | 4e-164 | 467 |
| LLPS-Sot-0033 | Solanum tuberosum | 92.86 | 4e-161 | 459 |
| LLPS-Nia-0818 | Nicotiana attenuata | 92.59 | 7e-164 | 466 |
| LLPS-Hea-0699 | Helianthus annuus | 92.12 | 8e-162 | 462 |
| LLPS-Viv-1001 | Vitis vinifera | 92.02 | 1e-160 | 458 |
| LLPS-Pot-1351 | Populus trichocarpa | 91.67 | 1e-162 | 463 |
| LLPS-Glm-0927 | Glycine max | 90.98 | 1e-163 | 466 |
| LLPS-Met-0316 | Medicago truncatula | 90.98 | 4e-164 | 467 |
| LLPS-Prp-0653 | Prunus persica | 90.83 | 4e-161 | 459 |
| LLPS-Phv-0093 | Phaseolus vulgaris | 90.5 | 1e-162 | 464 |
| LLPS-Cus-0443 | Cucumis sativus | 90.09 | 4e-154 | 442 |
| LLPS-Php-2173 | Physcomitrella patens | 89.41 | 4e-154 | 442 |
| LLPS-Mua-0032 | Musa acuminata | 89.39 | 1e-163 | 464 |
| LLPS-Vir-1227 | Vigna radiata | 88.62 | 7e-162 | 461 |
| LLPS-Via-0462 | Vigna angularis | 88.62 | 2e-162 | 462 |
| LLPS-Brd-0560 | Brachypodium distachyon | 88.43 | 7e-159 | 453 |
| LLPS-Tra-2712 | Triticum aestivum | 88.28 | 1e-154 | 442 |
| LLPS-Tru-0424 | Triticum urartu | 88.07 | 3e-158 | 449 |
| LLPS-Thc-0601 | Theobroma cacao | 87.36 | 2e-46 | 160 |
| LLPS-Ori-0251 | Oryza indica | 87.35 | 5e-159 | 454 |
| LLPS-Ors-1204 | Oryza sativa | 87.35 | 6e-160 | 454 |
| LLPS-Org-0670 | Oryza glaberrima | 87.35 | 5e-159 | 454 |
| LLPS-Orm-0205 | Oryza meridionalis | 87.35 | 5e-159 | 454 |
| LLPS-Orr-0004 | Oryza rufipogon | 87.35 | 5e-159 | 454 |
| LLPS-Amt-0479 | Amborella trichopoda | 87.3 | 2e-157 | 447 |
| LLPS-Orgl-0468 | Oryza glumaepatula | 86.94 | 3e-159 | 452 |
| LLPS-Orbr-0501 | Oryza brachyantha | 86.94 | 1e-159 | 453 |
| LLPS-Orni-0943 | Oryza nivara | 86.72 | 1e-154 | 442 |
| LLPS-Orb-1091 | Oryza barthii | 86.72 | 1e-154 | 442 |
| LLPS-Sob-0566 | Sorghum bicolor | 86.72 | 1e-155 | 445 |
| LLPS-Sei-0192 | Setaria italica | 86.61 | 1e-154 | 442 |
| LLPS-Zem-0307 | Zea mays | 86.45 | 2e-130 | 392 |
| LLPS-Hov-0051 | Hordeum vulgare | 86.36 | 3e-154 | 442 |
| LLPS-Xet-0542 | Xenopus tropicalis | 81.51 | 1e-135 | 395 |
| LLPS-Sem-0513 | Selaginella moellendorffii | 81.48 | 2e-143 | 414 |
| LLPS-Pes-0361 | Pelodiscus sinensis | 81.01 | 3e-135 | 392 |
| LLPS-Scf-0083 | Scleropages formosus | 80.59 | 2e-132 | 387 |
| LLPS-Osl-0256 | Ostreococcus lucimarinus | 80.42 | 2e-141 | 407 |
| LLPS-Anc-1069 | Anolis carolinensis | 80.33 | 6e-134 | 390 |
| LLPS-Ora-0287 | Ornithorhynchus anatinus | 80.26 | 6e-129 | 375 |
| LLPS-Leo-0215 | Lepisosteus oculatus | 80.25 | 8e-87 | 265 |
| LLPS-Myl-3944 | Myotis lucifugus | 79.9 | 2e-110 | 326 |
| LLPS-Tar-0017 | Takifugu rubripes | 79.75 | 1e-132 | 387 |
| LLPS-Orn-1194 | Oreochromis niloticus | 79.75 | 6e-132 | 386 |
| LLPS-Asm-1656 | Astyanax mexicanus | 79.73 | 2e-123 | 365 |
| LLPS-Pof-1739 | Poecilia formosa | 79.41 | 1e-129 | 379 |
| LLPS-Ten-0293 | Tetraodon nigroviridis | 79.41 | 7e-133 | 388 |
| LLPS-Mod-1195 | Monodelphis domestica | 79.34 | 4e-135 | 394 |
| LLPS-Orl-0117 | Oryzias latipes | 79.32 | 1e-131 | 386 |
| LLPS-Urm-0228 | Ursus maritimus | 79.3 | 2e-126 | 373 |
| LLPS-Nol-0508 | Nomascus leucogenys | 79.24 | 6e-133 | 388 |
| LLPS-Scm-0527 | Scophthalmus maximus | 78.99 | 2e-130 | 382 |
| LLPS-Sah-1414 | Sarcophilus harrisii | 78.93 | 2e-134 | 392 |
| LLPS-Chr-0405 | Chlamydomonas reinhardtii | 78.72 | 3e-130 | 381 |
| LLPS-Ova-1702 | Ovis aries | 78.66 | 1e-133 | 391 |
| LLPS-Bot-0133 | Bos taurus | 78.66 | 2e-134 | 392 |
| LLPS-Gaa-0506 | Gasterosteus aculeatus | 78.6 | 7e-132 | 385 |
| LLPS-Dar-0001 | Danio rerio | 78.57 | 1e-130 | 382 |
| LLPS-Xim-0357 | Xiphophorus maculatus | 78.39 | 1e-129 | 381 |
| LLPS-Scc-0286 | Schizosaccharomyces cryophilus | 78.26 | 8e-131 | 382 |
| LLPS-Gog-0544 | Gorilla gorilla | 78.01 | 2e-134 | 392 |
| LLPS-Caj-0511 | Callithrix jacchus | 78.01 | 2e-133 | 392 |
| LLPS-Mea-0465 | Mesocricetus auratus | 78.01 | 2e-134 | 392 |
| LLPS-Mum-0199 | Mus musculus | 78.01 | 2e-134 | 392 |
| LLPS-Maf-0468 | Macaca fascicularis | 78.01 | 2e-134 | 392 |
| LLPS-Chs-0206 | Chlorocebus sabaeus | 78.01 | 1e-134 | 392 |
| LLPS-Aon-0524 | Aotus nancymaae | 78.01 | 1e-134 | 391 |
| LLPS-Cea-0121 | Cercocebus atys | 78.01 | 2e-134 | 392 |
| LLPS-Sus-0865 | Sus scrofa | 78.01 | 7e-135 | 393 |
| LLPS-Ran-0659 | Rattus norvegicus | 78.01 | 9e-135 | 393 |
| LLPS-Paa-0942 | Papio anubis | 78.01 | 2e-134 | 392 |
| LLPS-Mal-1231 | Mandrillus leucophaeus | 78.01 | 5e-135 | 393 |
| LLPS-Aim-0232 | Ailuropoda melanoleuca | 78.01 | 2e-134 | 393 |
| LLPS-Dio-0060 | Dipodomys ordii | 78.01 | 8e-135 | 392 |
| LLPS-Rhb-0021 | Rhinopithecus bieti | 78.01 | 5e-135 | 394 |
| LLPS-Pap-0005 | Pan paniscus | 78.01 | 4e-134 | 392 |
| LLPS-Pat-0536 | Pan troglodytes | 78.01 | 8e-134 | 391 |
| LLPS-Hos-1682 | Homo sapiens | 78.01 | 1e-134 | 392 |
| LLPS-Fec-0085 | Felis catus | 78.01 | 1e-134 | 392 |
| LLPS-Man-0753 | Macaca nemestrina | 78.01 | 1e-134 | 393 |
| LLPS-Cas-0093 | Carlito syrichta | 78.01 | 1e-133 | 391 |
| LLPS-Caf-0024 | Canis familiaris | 78.01 | 1e-134 | 392 |
| LLPS-Poa-0341 | Pongo abelii | 78.01 | 2e-134 | 392 |
| LLPS-Eqc-0186 | Equus caballus | 78.01 | 2e-134 | 392 |
| LLPS-Mam-0271 | Macaca mulatta | 78.01 | 1e-134 | 392 |
| LLPS-Scp-0505 | Schizosaccharomyces pombe | 77.92 | 1e-131 | 384 |
| LLPS-Orc-0472 | Oryctolagus cuniculus | 77.69 | 9e-134 | 388 |
| LLPS-Fud-0901 | Fukomys damarensis | 77.59 | 2e-133 | 389 |
| LLPS-Otg-0083 | Otolemur garnettii | 77.59 | 3e-134 | 393 |
| LLPS-Loa-1848 | Loxodonta africana | 77.59 | 1e-133 | 390 |
| LLPS-Ict-2737 | Ictidomys tridecemlineatus | 77.59 | 6e-133 | 388 |
| LLPS-Cap-0179 | Cavia porcellus | 77.59 | 3e-133 | 389 |
| LLPS-Abg-1691 | Absidia glauca | 77.49 | 2e-128 | 377 |
| LLPS-Cis-0044 | Ciona savignyi | 77.45 | 4e-130 | 380 |
| LLPS-Icp-0629 | Ictalurus punctatus | 77.41 | 1e-125 | 369 |
| LLPS-Scj-0411 | Schizosaccharomyces japonicus | 77.39 | 8e-131 | 382 |
| LLPS-Drm-0625 | Drosophila melanogaster | 77.35 | 6e-129 | 379 |
| LLPS-Cii-0412 | Ciona intestinalis | 76.89 | 7e-131 | 382 |
| LLPS-Tut-0252 | Tursiops truncatus | 76.76 | 2e-130 | 382 |
| LLPS-Cae-0032 | Caenorhabditis elegans | 76.09 | 1e-121 | 360 |
| LLPS-Miv-0330 | Microbotryum violaceum | 75.42 | 5e-127 | 374 |
| LLPS-Put-0466 | Puccinia triticina | 75.42 | 3e-126 | 371 |
| LLPS-Spr-0412 | Sporisorium reilianum | 75.21 | 3e-124 | 367 |
| LLPS-Mup-1997 | Mustela putorius furo | 74.9 | 5e-130 | 379 |
| LLPS-Usm-0412 | Ustilago maydis | 74.69 | 2e-124 | 367 |
| LLPS-Mel-0300 | Melampsora laricipopulina | 74.58 | 3e-125 | 369 |
| LLPS-Pyt-0225 | Pyrenophora teres | 74.58 | 9e-126 | 367 |
| LLPS-Mao-0018 | Magnaporthe oryzae | 74.48 | 2e-123 | 364 |
| LLPS-Pug-1216 | Puccinia graminis | 74.27 | 2e-126 | 372 |
| LLPS-Phn-0667 | Phaeosphaeria nodorum | 74.07 | 1e-123 | 365 |
| LLPS-Pytr-0071 | Pyrenophora triticirepentis | 73.93 | 5e-120 | 355 |
| LLPS-Map-0053 | Magnaporthe poae | 73.62 | 4e-121 | 355 |
| LLPS-Yal-0166 | Yarrowia lipolytica | 73.62 | 7e-122 | 360 |
| LLPS-Blg-0234 | Blumeria graminis | 73.33 | 2e-123 | 364 |
| LLPS-Sac-0223 | Saccharomyces cerevisiae | 73.31 | 3e-122 | 361 |
| LLPS-Kop-0040 | Komagataella pastoris | 73.31 | 2e-121 | 358 |
| LLPS-Gas-0531 | Galdieria sulphuraria | 73.14 | 5e-128 | 376 |
| LLPS-Tum-0500 | Tuber melanosporum | 72.97 | 3e-52 | 174 |
| LLPS-Asn-0458 | Aspergillus nidulans | 72.88 | 9e-120 | 354 |
| LLPS-Ast-0163 | Aspergillus terreus | 72.88 | 4e-120 | 355 |
| LLPS-Gag-0166 | Gaeumannomyces graminis | 72.8 | 3e-121 | 358 |
| LLPS-Scs-0344 | Sclerotinia sclerotiorum | 72.8 | 3e-122 | 361 |
| LLPS-Asni-0096 | Aspergillus niger | 72.57 | 1e-120 | 357 |
| LLPS-Dos-0096 | Dothistroma septosporum | 72.54 | 1e-122 | 362 |
| LLPS-Asg-1073 | Ashbya gossypii | 72.46 | 5e-121 | 358 |
| LLPS-Zyt-0666 | Zymoseptoria tritici | 72.36 | 2e-122 | 362 |
| LLPS-Crn-0258 | Cryptococcus neoformans | 72.27 | 7e-121 | 357 |
| LLPS-Nef-0686 | Neosartorya fischeri | 71.73 | 4e-119 | 353 |
| LLPS-Asc-0817 | Aspergillus clavatus | 71.31 | 2e-118 | 351 |
| LLPS-Asfu-0390 | Aspergillus fumigatus | 71.31 | 3e-118 | 350 |
| LLPS-Nec-1004 | Neurospora crassa | 71.01 | 1e-117 | 349 |
| LLPS-Asf-0262 | Aspergillus flavus | 70.64 | 7e-119 | 352 |
| LLPS-Aso-0161 | Aspergillus oryzae | 70.64 | 7e-119 | 352 |
| LLPS-Chc-0088 | Chondrus crispus | 70.61 | 1e-123 | 365 |
| LLPS-Fus-0772 | Fusarium solani | 69.67 | 9e-118 | 349 |
| LLPS-Trr-0168 | Trichoderma reesei | 68.85 | 3e-118 | 351 |
| LLPS-Fuv-0100 | Fusarium verticillioides | 68.75 | 6e-114 | 340 |
| LLPS-Beb-0302 | Beauveria bassiana | 68.72 | 6e-117 | 348 |
| LLPS-Cog-0447 | Colletotrichum gloeosporioides | 68.49 | 1e-115 | 343 |
| LLPS-Cogr-0211 | Colletotrichum graminicola | 68.35 | 5e-114 | 340 |
| LLPS-Coo-0617 | Colletotrichum orbiculare | 67.51 | 6e-113 | 338 |
| LLPS-Trv-0128 | Trichoderma virens | 67.48 | 2e-117 | 346 |
| LLPS-Ved-0331 | Verticillium dahliae | 67.09 | 9e-112 | 334 |
| LLPS-Cym-0303 | Cyanidioschyzon merolae | 66.8 | 3e-121 | 360 |
| LLPS-Lac-1094 | Latimeria chalumnae | 61.67 | 2e-90 | 280 |
| LLPS-Lep-0210 | Leersia perrieri | 59.58 | 7e-83 | 268 |
| LLPS-Dac-0048 | Daucus carota | 54.1 | 2e-69 | 223 |
| LLPS-Orp-0331 | Oryza punctata | 49.39 | 2e-62 | 205 |