※ Scaffold introduction

    Scaffolds were defined as the drivers of LLPS essential for the structural integrity of MLOs, and the major components which, alone or with co-scaffolds, undergo LLPS (1-4). A minimum set of six experimental tests, including the assembly of spherical droplets, the observation of fusion events, and the identification of mutations that abolish or inhibit LLPS in vitro and in cells have been proposed for rigorous analysis of LLPS processes (1). For each known scaffold protein, descriptions on performed assays of the minimum set of experiments were presented on its gene page. For example, the human fused in sarcoma (FUS), a well-characterized RNA-binding protein undergoing LLPS involved in formation of multiple biomolecular condensates (5,6-8), forms liquid-like droplets both in cells and at near physiological conditions in vitro. All the six experimental tests were performed to analyze the LLPS properties of FUS (9).

References
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