※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Put-0539	PTTG_06589	A0A0C4F0H2	ADK1
2		LLPS-Put-0293	PTTG_00214	A0A180GYW5	PTTG_00214
3		LLPS-Put-0302	PTTG_00256	A0A180G016	PTTG_00256
4		LLPS-Put-1329	PTTG_00298	A0A180H1J2	PTTG_00298
5		LLPS-Put-0428	PTTG_00443	A0A180H5V4	PTTG_00443
6		LLPS-Put-1109	PTTG_01343	A0A0C4EKR6	PTTG_01343
7		LLPS-Put-0751	PTTG_01432	A0A180H4N5	PTTG_01432
8		LLPS-Put-0466	PTTG_01649	A0A0C4ELL5	PTTG_01649
9		LLPS-Put-0290	PTTG_01940	A0A180GBS5	PTTG_01940
10		LLPS-Put-1082	PTTG_02247	A0A180GIF6	PTTG_02247
11		LLPS-Put-0331	PTTG_02573	A0A180GGT4	PTTG_02573
12		LLPS-Put-0305	PTTG_03432	A0A180GV81	PTTG_03432
13		LLPS-Put-0257	PTTG_03545	A0A0C4ERX5	PTTG_03545
14		LLPS-Put-0006	PTTG_04289	A0A0C4EU10	PTTG_04289
15		LLPS-Put-0210	PTTG_04330	A0A180GNB1	PTTG_04330
16		LLPS-Put-0504	PTTG_04392	A0A180GNL1	PTTG_04392
17		LLPS-Put-0303	PTTG_04539	A0A0C4EUQ8	PTTG_04539
18		LLPS-Put-0391	PTTG_05898	A0A180GYE4	PTTG_05898
19		LLPS-Put-0516	PTTG_06273	A0A0C4EZL4	PTTG_06273
20		LLPS-Put-0265	PTTG_07702	A0A180H1N3	PTTG_07702
21		LLPS-Put-0486	PTTG_08056	A0A0C4F4L6	PTTG_08056
22		LLPS-Put-0736	PTTG_08086	A0A180GVU3	PTTG_08086
23		LLPS-Put-0765	PTTG_08360	A0A180GC17	PTTG_08360
24		LLPS-Put-1330	PTTG_09114	A0A180G2R5	PTTG_09114
25		LLPS-Put-0141	PTTG_10312	A0A180G3B5	PTTG_10312
26		LLPS-Put-0422	PTTG_05333	A0A180H2S3	PTTG_25520
27		LLPS-Put-0102	PTTG_00769	A0A180GQ90	PTTG_26852
28		LLPS-Put-1255	PTTG_01640	A0A180GMJ4	PTTG_27168